Co-culture with endothelial progenitor cells promotes survival, migration, and differentiation of osteoclast precursors
Co-culture with endothelial progenitor cells promotes survival, migration, and differentiation of osteoclast precursors
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与内皮祖细胞共培养可促进破骨细胞前体的存活、迁移和分化
DOI：
10.1016/j.bbrc.2012.11.081
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发表时间：
2013-01-11
影响因子：
3.1
通讯作者：
Xu, Jian-Zhong
中科院分区：
文献类型：
--
作者：
Pang, Hao;Wu, Xue-Hui;Xu, Jian-Zhong
本研究通过建立小鼠内皮祖细胞（endothelial progenitor cells，EPCs）与RAW 264.7单核细胞间接共培养体系，观察EPCs对破骨细胞前体细胞生物学行为的影响。结果表明，破骨细胞前体细胞与EPCs共培养后，其存活、迁移和分化能力均得到显著增强。这些表型变化与影响细胞行为的多个基因的上调相一致，包括磷酸-VEGFR-2、CXCR 4、磷酸-Smad 2/3、磷酸-Akt、磷酸-ERK 1和磷酸-p38 MAPK。这些结果共同表明，EPCs可以调节破骨细胞前体的存活，迁移和分化潜力，从而为理解血管生成和骨稳态之间的相关性提供了新的见解。(C)2012 Elsevier Inc. All rights reserved.
In this study, we report the effect of endothelial progenitor cells (EPCs) on the biological behavior of osteoclast precursors in vitro by establishing an indirect co-culture system of mice EPCs and RAW 264.7 monocyte cells. Results show that the survival, migration, and differentiation of osteoclast precursors were greatly enhanced when co-cultured with EPCs. These phenotypic changes coincide with the upregulation of multiple genes affected cell behavior, including phospho-VEGFR-2, CXCR4, phospho-Smad2/3, phospho-Akt, phospho-ERK1, and phospho-p38 MAPK. The results collectively suggest that EPCs could modulate the survival, migration, and differentiation potential of osteoclast precursors, thus providing new insights in understanding of correlation between angiogenesis and bone homeostasis. (C) 2012 Elsevier Inc. All rights reserved.