Functional characterization of a new metallochaperone for reducing cadmium concentration in rice crop
Functional characterization of a new metallochaperone for reducing cadmium concentration in rice crop
复制标题
降低水稻镉浓度的新型金属伴侣的功能表征
DOI：
10.1016/j.jclepro.2020.123152
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发表时间：
2020-11-01
影响因子：
11.1
通讯作者：
Yang, Zhi Min
中科院分区：
文献类型：
--
作者：
Khan, Irfan Ullah;Rono, Justice Kipkorir;Yang, Zhi Min
植物金属伴侣蛋白是一类特异性的金属结合蛋白，介导金属的分配和稳态，但其生物学功能及其在金属获取、转运和解毒中的作用机制尚不清楚。本研究对一个新的重金属相关异戊二烯化植物蛋白（HIPP）亚家族成员OsHIPP 42进行了功能鉴定，OsHIPP 42与水稻对镉的耐性有关。OsHIPP 42蛋白靶向细胞核和质膜。它在整个生命周期中在节、基茎和花序梗中转录表达。过量Cd、Zn、Mn和Cu胁迫下，OsHIPP 42在根中被充分诱导。在表达OsHIPP 42的酵母细胞中证明了在Cd胁迫期间的耐受能力。过量表达（OX）OsHIPP 42的转基因水稻表现出提高的植株伸长、生物量和叶绿素积累。相比之下，由于T-DNA插入导致的OsHIPP 42敲除突变导致受损的生长反应。有趣的是，Oshipp 42 -1和OsHIPP 42 -1突变体都降低了稻草中的Cd浓度，特别是在谷粒中，比野生型低38.6-44.6%。值得注意的是，OsHIPP 42过表达和突变体系未被发现参与水稻对过量Zn、Mn和Cu的耐受，表明OsHIPP 42可能选择性地在Cd分配和解毒中起作用。这项工作强调了遗传改良基因型材料的重要性，这些材料将有可能用于最大限度地减少水稻作物中Cd的积累，并通过食物链减少环境对人类健康的风险。(C)2020爱思唯尔有限公司版权所有。
Plant metallochaperones are a class of specific metal-binding proteins that mediate metal distribution and homeostasis, but their biological functions and mechanisms underlying metal acquisition, translocation and detoxification are largely unknown. This study functionally characterized a novel heavy metal-associated isoprenylated plant protein (HIPP) subfamily member OsHIPP42 involved in rice tolerance to the toxic metal cadmium (Cd). The OsHIPP42 proteins were targeted to the nucleus and plasma membrane. It was transcriptionally expressed in nodes, basal stems and peduncles throughout the life span. OsHIPP42 was sufficiently induced in roots under excess Cd, zinc (Zn), manganese (Mn) and copper (Cu) stresses. The tolerant capability during Cd stress was demonstrated in the yeast cells expressing OsHIPP42. Transgenic rice overexpressing (OX) OsHIPP42 displayed improved plant elongation, biomass, and chlorophyll accumulation. In contrast, the OsHIPP42 knockout mutation due to the T-DNA insertion resulted in compromised growth response. Intriguingly, both Oshipp42-1 and OsHIPP42-1 mutant lines had a reduced Cd concentration in rice straw, particularly in the grains, with 38.6-44.6% lower than those of wild-type. Notably, the OsHIPP42 overexpression and mutant lines were not found to be involved in rice tolerance to excess Zn, Mn and Cu, suggesting that OsHIPP42 may work selectively on the Cd allocation and detoxification. This work highlights the significance of the genetically improved genotype materials that will potentially be used to minimize Cd accumulation in rice crop and reduce environmental risks to human health through food chains. (C) 2020 Elsevier Ltd. All rights reserved.