Conventional capillary electrophoresis in comparison with short-capillary capillary electrophoresis and microfabricated glass chip capillary electrophoresis for the analysis of fluorescein isothiocyanate anti-human immunoglobulin G.
Conventional capillary electrophoresis in comparison with short-capillary capillary electrophoresis and microfabricated glass chip capillary electrophoresis for the analysis of fluorescein isothiocyanate anti-human immunoglobulin G.
复制标题
传统毛细管电泳与短毛细管毛细管电泳和微型玻璃芯片毛细管电泳分析异硫氰酸荧光素抗人免疫球蛋白 G 的比较。
DOI：
10.1016/s0021-9673(97)00667-5
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
S. F. Li
中科院分区：
文献类型：
--
作者：
I. Rodríguez;Y. Zhang;H. K. Lee;S. F. Li
在短毛细管和微加工玻璃芯片中实现了异硫氰酸荧光素抗人IgG的快速，有效的分析。毛细管从进样端到检测器的长度为6cm，电场强度为0.268kV/cm。在1分钟内进行分析。使用标准的光刻程序和化学湿法蚀刻制造玻璃微芯片装置。使用直接粘合技术密封通道。对于2.8 cm的有效长度和0.526 kV/cm的电场强度，在不到16 s内实现电泳分析。传统的毛细管电泳得到了高度重复性的结果和良好的检测限。分析时间为2.5分钟的毛细管与47厘米的有效长度和电场强度为0.383千伏/厘米。
Fast, efficient analysis of fluorescein isothiocyanate anti-human IgG was achieved in a short-capillary and in a microfabricated glass chip. The capillary was 6 cm long from injection end to detector with electric field strength of 0.268 kV/cm. Analysis were performed within 1 min. A glass microchip device was fabricated using standard photolithographic procedures and chemical wet etching. The channels were sealed using a direct bonding technique. For an effective length of 2.8 cm with electric field strength of 0.526 kV/cm, electrophoretic analysis was achieved in less than 16 s. Conventional capillary electrophoresis gave highly reproducible results and good detection limits. Analysis time was 2.5 min for a capillary with 47 cm effective length and electric field strength of 0.383 kV/cm.