GABAergic Neurons Are Less Selective to Stimulus Orientation than Excitatory Neurons in Layer II/III of Visual Cortex, as Revealed by In Vivo Functional Ca2+ Imaging in Transgenic Mice
GABAergic Neurons Are Less Selective to Stimulus Orientation than Excitatory Neurons in Layer II/III of Visual Cortex, as Revealed by In Vivo Functional Ca2+ Imaging in Transgenic Mice
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DOI：
10.1523/jneurosci.4641-06.2007
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发表时间：
2007-02
期刊：
影响因子：
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通讯作者：
K. Sohya;Katsuro Kameyama;Y. Yanagawa;K. Obata;T. Tsumoto
中科院分区：
文献类型：
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作者：
K. Sohya;Katsuro Kameyama;Y. Yanagawa;K. Obata;T. Tsumoto
视觉皮质中的大多数神经元对窄范围方向的视觉刺激有选择性反应，GABA能神经元被认为在这种方向选择性的形成中发挥作用。这表明GABA能神经元的反应特性可能不同于兴奋性神经元。然而，这一观点尚未得到证实。为了解决这个问题，我们在体内应用双光子功能钙离子成像转基因小鼠，其中GABA能神经元表达增强的绿色荧光蛋白。星形胶质细胞用星形胶质细胞特异性染料染色。三种类型的细胞，GABA能神经元，兴奋性神经元和星形胶质细胞，在第II/III层的视觉皮层的差异，通过使用不同波长的激发光和发射荧光的分色镜，并在不同的方向移动的视觉刺激的反应进行了测定与钙离子敏感染料的荧光强度的变化。我们发现几乎所有的GABA能神经元都具有方向不敏感反应，而大多数兴奋性神经元具有方向选择反应。
Most neurons in the visual cortex are selectively responsive to visual stimulation of a narrow range of orientations, and GABAergic neurons are considered to play a role in the formation of such orientation selectivity. This suggests that response properties of GABAergic neurons may be different from those of excitatory neurons. This view remains unproved, however. To address this issue, we applied in vivo two-photon functional Ca2+ imaging to transgenic mice, in which GABAergic neurons express enhanced green fluorescent protein. Astroglia were stained by an astrocyte-specific dye. The three types of cells, GABAergic neurons, excitatory neurons, and astrocytes, in layer II/III of the visual cortex were differentially identified by using different wavelengths of excitation light and a dichroic mirror for emitted fluorescence, and their responses to moving visual stimuli at different orientations were measured with changes in the intensity of fluorescence of a Ca2+-sensitive dye. We found that almost all GABAergic neurons have orientation-insensitive responses, whereas most of excitatory neurons have orientation-selective responses.