TISSUE ENGINEERING SCIENCE - CONSEQUENCES OF CELL TRACTION FORCE
TISSUE ENGINEERING SCIENCE - CONSEQUENCES OF CELL TRACTION FORCE
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DOI：
10.1007/bf00146673
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发表时间：
1992-01-01
期刊：
影响因子：
2.2
通讯作者：
MOON, AG
中科院分区：
文献类型：
--
作者：
TRANQUILLO, RT;DURRANI, MA;MOON, AG
在与生物医学和生物技术相关的各种情况下，血液和组织细胞与软组织和组织等效的重构胶原凝胶机械地相互作用。这些相互作用中的一个关键现象是细胞对局部胶原纤维施加牵引力，局部胶原纤维通常构成这些组织和凝胶的固体网络并赋予总体机械完整性。细胞对这种胶原网络施加牵引力的两个重要结果是，首先，当细胞协调它们的牵引力时，导致细胞迁移，其次，当它们的牵引力足以使网络变形时。这种细胞-胶原网络相互作用以多种方式偶联。例如，网络变形可导致胶原纤维的净排列，引起接触引导，其中细胞沿纤维排列的轴沿着双向偏置移动，潜在地导致不均匀的细胞分布。这可以控制伤口中的细胞积累，并被用来控制生物人工组织和器官的细胞浸润。细胞牵引的另一个结果是在调节细胞蛋白质和DNA合成和分化的网络中产生的应力和应变。我们总结，在这里，相关的数学理论，我们已经用来描述的内在耦合的细胞动力学和组织力学的细胞填充胶原蛋白凝胶通过牵引。适当的模型的发展基于这些理论，在努力了解伤口愈合的事件如何管理伤口收缩的速度和程度，并在体外测量细胞牵引力，进行了描述。相关的观察和推测，从细胞生物学和医学的动机或服务于批判的假设，在理论和模型也进行了总结。
Blood and tissue cells mechanically interact with soft tissues and tissue-equivalent reconstituted collagen gels in a variety of situations relevant to biomedicine and biotechnology. A key phenomenon in these interactions is the exertion of traction force by cells on local collagen fibers which typically constitute the solid network of these tissues and gels and impart gross mechanical integrity. Two important consequences of cells exerting traction on such collagen networks are first, when the cells co-ordinate their traction, resulting in cell migration, and second, when their traction is sufficient to deform the network. Such cell-collagen network interactions are coupled in a number of ways. Network deformation, for example, can result in net alignment of collagen fibers, eliciting contact guidance, wherein cells move with bidirectional bias along an axis of fiber alignment, potentially leading to a nonuniform cell distribution. This may govern cell accumulation in wounds and be exploited to control cell infiltration of bioartificial tissues and organs. Another consequence of cell traction is the resultant stress and strain in the network which modulate cell protein and DNA synthesis and differentiation. We summarize, here, relevant mathematical theories which we have used to describe the inherent coupling of cell dynamics and tissue mechanics in cell-populated collagen gels via traction. The development of appropriate models based on these theories, in an effort to understand how events in wound healing govern the rate and extent of wound contraction, and to measure cell traction forces in vitro, are described. Relevant observations and speculation from cell biology and medicine that motivate or serve to critique the assumptions made in the theories and models are also summarized.