Transcriptome analysis of Antarctic Rhodococcus sp. NJ-530 in the response to dimethylsulfoniopropionate
Transcriptome analysis of Antarctic Rhodococcus sp. NJ-530 in the response to dimethylsulfoniopropionate
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DOI：
10.1007/s00300-022-03049-w
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发表时间：
2022-06
期刊：
影响因子：
1.7
通讯作者：
Liping Zhang;Xixi Wang;Fushan Chen;Wenyu Wang;Chang-feng Qu;J. Miao
中科院分区：
文献类型：
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作者：
Liping Zhang;Xixi Wang;Fushan Chen;Wenyu Wang;Chang-feng Qu;J. Miao
二甲基磺基丙酸酯 (DMSP) 和二甲硫醚 (DMS) 是全球硫循环和气候调节中的关键分子。细菌 DMSP 依赖性 DMS 生产是 DMS 的重要天然来源。本工作的目的是利用RNA-seq技术研究南极红球菌基因表达谱的变化。 NJ-530对DMSP的反应，以及DMSP代谢的可能机制。在这项研究中，红球菌属。 NJ-530 细胞暴露于 0（对照）和 1 mM DMSP 9 小时。结果表明，DMSP 诱导红球菌属 sp 的转录变化。新泽西-530。 DMSP 导致多个基因上调，这些基因可能在 DMSP 代谢中发挥潜在作用。此外，DMSP 代谢的转录组分析确定了两种治疗策略之间总共 1159 个差异表达基因 (DEG)。进行 qRT-PCR 以进一步确认这些结果的变化。 GO和KEGG富集分析表明，DMS的产生是多个DMSP裂解相关基因沿着一条途径协调作用的结果，包括吸收机制、次级代谢和细胞信号传导。总之，我们的数据表明，DMSP 暴露诱导了 DMSP 裂解途径活性，并且在 DMSP 降解过程中在转录水平上分析了对菌株 NJ-530 基因表达谱的影响。这项工作深入了解了红球菌属的转录特征。 NJ-530 响应 DMSP，有助于阐明 DMSP 在红球菌中的生物降解和潜在机制。新泽西-530。
Dimethylsulfoniopropionate (DMSP) and dimethyl sulfide (DMS) are critical molecules in the global sulfur cycle and in climate regulation. Bacterial DMSP-dependent DMS production is an important natural source of DMS. The aim of the present work was to use RNA-seq technology to investigate the changes in gene expression profiles of AntarcticRhodococcussp. NJ-530 in response to DMSP, and the possible mechanism of DMSP metabolism. In this study,Rhodococcussp. NJ-530 cells were exposed to 0 (control) and 1 mM DMSP for 9 h. The results showed that DMSP induces transcriptional changes toRhodococcussp. NJ-530. DMSP caused upregulation of several genes that may play a potential role in DMSP metabolization. Additionally, transcriptome analysis of DMSP metabolism determinized a total of 1159 differentially expressed genes (DEGs) between two treatment strategies. qRT-PCR was performed to further confirm the changes in these results. GO and KEGG enrichment analyses showed that DMS production is the result of the coordinated action of a multitude of DMSP lysis-associated genes along a pathway, including absorption mechanism, secondary metabolisms, and cellular signaling. In conclusion, our data indicated that DMSP exposure induced DMSP cleavage pathway activity, and the effects on the gene expression profile of strain NJ-530 were analyzed at the transcriptional level during the degradation of DMSP. This work provided insight into the transcriptional characterization ofRhodococcussp. NJ-530 in response to DMSP and contributed to clarifying the biodegradation and underlying mechanism of DMSP inRhodococcussp. NJ-530.