Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus
Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus
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DOI：
10.1016/0192-0561(96)00028-8
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发表时间：
1996-05-01
期刊：
影响因子：
--
通讯作者：
Yoo, ID
中科院分区：
文献类型：
--
作者：
Kim, HM;Han, SB;Yoo, ID
从桑黄(Pellinus linteus，PL)菌丝体培养物中提纯多糖，观察其对正常脾细胞免疫功能的影响。总体而言，在小鼠脾淋巴细胞培养和腹腔注射中加入磷脂酰胆碱均能增强小鼠的体内外免疫功能。注射给小鼠，而S-葡聚糖等多糖只提高T淋巴细胞介导的免疫水平。PL刺激T淋巴细胞的免疫功能，如混合淋巴细胞反应诱导的T淋巴细胞增殖和细胞毒T细胞对同种异体抗原的细胞毒作用。在体内和体外，PL均能增强自然杀伤细胞和巨噬细胞介导的非特异性免疫功能。PL还能刺激体液免疫功能，如T依赖和T非依赖的一抗反应，并可作为B细胞的多克隆激活剂。与担子菌中分离的其他多糖相比，PL具有更广泛的免疫刺激和抗肿瘤活性。版权所有(C)1996国际免疫药理学会
Polysaccharide was purified from mycelial culture of Phellinus linteus (PL) and its effect on immunocompetence of normal splenocytes was observed. Overall in vitro and in vivo immune function was enhanced by addition of PL into culture of mouse spleen lymphocyte and I.p. injection into mouse, while S-glucans and other polysaccharides only raised the level of T lymphocyte-mediated immunity. PL stimulated immune functions of T lymphocytes, such as proliferation of T lymphocyte induced by mixed lymphocyte reaction and cytotoxicity of cytotoxic T cells responding to alloantigen. Nonspecific immune functions mediated by natural killer cells and macrophages were increased by treatment of PL in vivo and in vitro. PL also stimulated humoral immune function positively, such as T-dependent and T-independent primary antibody responses, and acted as a polyclonal activator on B cell. PL exhibited a wider range of immunostimulation and antitumor activity than other polysaccharides isolated from Basidiomycetes. Copyright (C) 1996 International Society for Immunopharmacology