Enhanced translation initiation factor 4G levels correlate with production levels of monoclonal antibodies in recombinant CHO cell lines.
Enhanced translation initiation factor 4G levels correlate with production levels of monoclonal antibodies in recombinant CHO cell lines.
复制标题
增强的翻译起始因子 4G 水平与重组 CHO 细胞系中单克隆抗体的生产水平相关。
DOI：
10.1042/bj20151314
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Pavitt GD
中科院分区：
文献类型：
--
作者：
Pavitt GD
使用细胞制造基于蛋白质的治疗药物或生物制药是一项快速扩张的工业活动。中国仓鼠卵巢 (CHO) 细胞是生物制药生产中最常用的哺乳动物宿主表达系统。在过去的 30 年里，学术和工业研究人员研究了细胞表达特征，旨在提高产品产量、质量、可扩展性和可重复性。尽管基因表达和分泌途径的许多步骤都得到了优化，但在此过程中优化蛋白质合成因子和调节因子却很少受到关注。 Mead 等人在《生化杂志》上进行的一项新研究首次对几种蛋白质合成因子进行了系统研究，发现 eIF4G1 的表达水平与培养物中表达的重组蛋白的水平相关。优化蛋白质合成因子的水平和活性可能有助于增强生物制药的重组蛋白表达。
Using cells to manufacture protein-based therapeutics or biopharmaceuticals is a rapidly expanding industrial activity. Chinese hamster ovary (CHO) cells are the most frequently used mammalian host-expression system for the manufacture of biopharmaceuticals. Over the past ∼30 years academic and industrial researchers have studied cell expression characteristics with aims to improve product yield, quality, scalability and reproducibility. Although many steps in the gene expression and secretion pathways have been optimized, little attention has been paid to optimizing protein synthesis factors and regulators during this process. A new study in Biochemical Journal by Mead et al., provides a first systematic study of several protein synthesis factors and finds that the expression level of eIF4G1 correlates with the level of recombinant protein expressed in cultures. Optimizing levels and activities of protein synthesis factors may help to enhance recombinant protein expression of biopharmaceuticals.
选择用于 cGMP 生产的重组 CHO 细胞系的策略：提高细胞系生成的效率
DOI：
--
发表时间：
2010
期刊：
Biotechnology progress (Print)
影响因子：
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作者：
Alison J. Porter;A. Racher;R. Preziosi;A. Dickson
通讯作者：
A. Dickson