Proteome Analysis of Roots in Cucumber Seedlings Under Iso-Osmotic NaCl and Ca(NO3)(2) Stresses
Proteome Analysis of Roots in Cucumber Seedlings Under Iso-Osmotic NaCl and Ca(NO3)(2) Stresses
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等渗 NaCl 和 Ca(NO3)(2) 胁迫下黄瓜幼苗根部蛋白质组分析
DOI：
10.1007/s11105-015-0916-4
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发表时间：
2016
影响因子：
2.1
通讯作者：
Sun Jin
中科院分区：
文献类型：
--
作者：
Shao Qiaosai;Shu Sheng;Du Jing;Yuan Yinghui;Xing Wenwen;Guo Shirong;Sun Jin
盐渍化严重影响黄瓜的生长和产量。为了解黄瓜幼苗对盐胁迫的复杂响应机制，研究了等渗NaCl和Ca（NO3）2胁迫对黄瓜幼苗生理生化的影响。在本研究中，等渗NaCl和Ca（NO3）2胁迫均显著降低了紫茎泽兰的生物量，且Ca（NO3）2胁迫对紫茎泽兰生物量的抑制作用小于NaCl胁迫。Ca（NO3）2胁迫下可溶性蛋白质含量增加，而NaCl胁迫6 d后可溶性蛋白质含量下降。SDS-聚丙烯酰胺凝胶电泳结果表明，等渗NaCl和Ca（NO3）2胁迫0、3、6、9 d时，根中有14条差异表达蛋白带，而NaCl胁迫6、9 d时，根中有7条差异表达蛋白带。在此基础上，采用双向凝胶电泳技术对等渗NaCl和Ca（NO3）2胁迫下黄瓜根系蛋白质进行了分离。在盐胁迫下，共有43个蛋白质点发生了变化。在这些蛋白质中，33个被成功地鉴定了基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）和液相色谱电喷雾电离串联质谱（LC-ESI-MS/MS），并分类，包括那些对应于抗氧化剂和防御相关蛋白质和能量和代谢。对差异表达蛋白的功能进行分析，有助于更好地理解不同盐胁迫的响应机制，表明黄瓜幼苗具有更强的抗Ca（NO3）2胁迫能力。
The growth and productivity of cucumber are severely affected by salinity. To understand the complex salt response mechanism, the physiological and biochemical responses of cucumber seedlings to iso-osmotic NaCl and Ca(NO3)2stresses were investigated. In this study, the biomass was significantly decreased under iso-osmotic NaCl and Ca(NO3)2stresses, and the inhibitory effect of Ca(NO3)2stress was less than that of NaCl stress. The soluble protein contents were increased under Ca(NO3)2stress, whereas they were decreased after 6 days of NaCl stress. A sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis revealed that there were 14 differentially expressed protein bands in roots under iso-osmotic NaCl and Ca(NO3)2stresses at 0, 3, 6, and 9 days, and seven protein bands were little expressed under NaCl stress at 6 and 9 days. Based on these results, 2-D gel electrophoresis was used to separate cucumber root proteins in response to iso-osmotic NaCl and Ca(NO3)2stresses at 3 days. A total of 43 protein spots changed under salt stress. Of these proteins, 33 were successfully identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography electro-spray ionization tandem mass spectrometry (LC-ESI-MS/MS) and categorized into classes, including those corresponding to antioxidants and defense-related proteins and energy and metabolism. The functions of the significantly differentially expressed root proteins were analyzed, which may facilitate a better understanding of different salt response mechanisms, and we suggest that cucumber seedlings showed a more powerful ability to resist Ca(NO3)2stress.