Direct tracheal instillation of solutes into mouse lung.
Direct tracheal instillation of solutes into mouse lung.
复制标题
DOI：
10.3791/1941
复制
发表时间：
2010-08-29
期刊：
影响因子：
--
通讯作者：
Rojas, Mauricio
中科院分区：
文献类型：
--
作者：
Helms, My N;Torres-Gonzalez, Edilson;Rojas, Mauricio
气管内滴注将溶质直接输送到肺部。该程序的目标是将灌注物输送到肺的远端区域，因此经常被纳入旨在研究肺泡的研究中。我们提供了一个详细的生存方案进行气管内滴注小鼠。使用这种方法，可以将测试溶质或固体（如肺治疗剂、表面活性剂、病毒和小寡核苷酸）靶向递送到远端肺。气管内滴注可能是首选方法，而吸入方案可能主要针对上呼吸道，并可能使研究人员暴露于潜在的有害物质。此外，在使用气管灌注方案时，动物可以从非侵入性手术中完全恢复。这允许对试验动物进行后续生理测量，或使用同一动物重新安装。为确保动物的康复，必须仔细确定注入肺部的剂量，并保持渗透平衡。通常情况下，可将30-75 μ l的灌注量引入小鼠肺。
Intratracheal instillations deliver solutes directly into the lungs. This procedure targets the delivery of the instillate into the distal regions of the lung, and is therefore often incorporated in studies aimed at studying alveoli. We provide a detailed survival protocol for performing intratracheal instillations in mice. Using this approach, one can target delivery of test solutes or solids (such as lung therapeutics, surfactants, viruses, and small oligonucleotides) into the distal lung. Tracheal instillations may be the preferred methodology, over inhalation protocols that may primarily target the upper respiratory tract and possibly expose the investigator to potentially hazardous substances. Additionally, in using the tracheal instillation protocol, animals can fully recover from the non-invasive procedure. This allows for making subsequent physiological measurements on test animals, or reinstallation using the same animal. The amount of instillate introduced into the lung must be carefully determined and osmotically balanced to ensure animal recovery. Typically, 30-75 muL instillate volume can be introduced into mouse lung.