Effects of Polyunsaturated Fatty Acids and Prostaglandins on Oocyte Maturation in a Marine Teleost, the European Sea Bass (Dicentrarchus labrax)1
Effects of Polyunsaturated Fatty Acids and Prostaglandins on Oocyte Maturation in a Marine Teleost, the European Sea Bass (Dicentrarchus labrax)1
复制标题
DOI：
10.1095/biolreprod64.1.382
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Lisa Ann Sorbera;Juan Francisco Asturiano;M. Carrillo;S. Zanuy
中科院分区：
文献类型：
--
作者：
Lisa Ann Sorbera;Juan Francisco Asturiano;M. Carrillo;S. Zanuy
摘要研究了多不饱和脂肪酸（PUFAs）、花生四烯酸（AA）、二十碳五烯酸（EPA）、二十二碳六烯酸（DHA）和前列腺素（PGs）对黑鲈卵母细胞成熟的影响。卵泡封闭的卵黄发生后，排卵前的卵母细胞进行体外培养和成熟进行了验证，通过评估体积增加，脂滴聚结，蛋黄澄清，和萌发囊泡迁移和故障。人绒毛膜促性腺激素作为促成熟促性腺激素（GTH）给药，能够以时间和剂量依赖性方式诱导成熟。游离AA以剂量和时间依赖性方式诱导成熟，并增强GTH诱导的成熟，而EPA、DHA和油酸则无效。GTH诱导的成熟被磷脂酶A2阻断剂显著抑制，表明AA的动员参与了GTH诱导的成熟。此外，EPA和DHA表现出显着的，剂量依赖性衰减GTH诱导的成熟。GTH诱导的成熟在环氧化酶抑制剂吲哚美辛的存在下被抑制，并且这种抑制被添加AA、PGE 2或PGF 2 α逆转。PGE 2和PGF 2 α单独使用均能有效促进成熟，而PGE 1和PGE 3则无效。PUFAs对卵母细胞体外成熟的影响与体内研究相证实。卵母细胞获得的女性喂养的商业，PUFA-丰富的饮食（RD）和成熟的行为进行了比较，从女性喂养的天然饮食（ND）具有较高的EPA含量和n-3：n-6的比例。虽然没有观察到显着差异的卵母细胞的自发成熟率，更高的百分比GTH诱导成熟和更低的比例闭锁在RD卵母细胞。此外，虽然两组卵母细胞的基础PGE产量相同，但在存在hCG的情况下，RD卵母细胞产生的PGE水平显着更高。本研究结果为AA代谢参与GTH诱导的卵母细胞成熟提供了证据，并提示其他PUFAs和PGs可能在海洋硬骨鱼卵母细胞成熟诱导中发挥重要作用。
Abstract The effects of the polyunsaturated fatty acids (PUFAs), arachidonic acid (AA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and prostaglandins (PGs) on oocyte maturation were investigated in a marine teleost, the sea bass (Dicentrarchus labrax). Follicle-enclosed postvitellogenic, preovulatory oocytes were cultured in vitro and maturation was verified by assessing volume increase, lipid droplet coalescence, yolk clarification, and germinal vesicle migration and breakdown. Human chorionic gonadotropin was administered as the maturation-inducing gonadotropin (GTH) and was capable of inducing maturation in a time- and dose-dependent manner. Free AA induced maturation in a dose- and time-dependent manner and enhanced GTH-induced maturation, while EPA, DHA, and oleic acid were ineffective. Maturation induced by GTH was significantly suppressed by a phospholipase A2 blocker, suggesting that mobilization of AA was involved in GTH-induced maturation. Moreover, EPA and DHA exhibited a significant, dose-dependent attenuation of GTH-induced maturation. Maturation induced by GTH was inhibited in the presence of a cyclooxygenase inhibitor, indomethacin, and this inhibition was reversed by addition of AA, PGE2, or PGF2α. PGE2 and PGF2α alone were both effective stimulators of maturation, while PGE1 and PGE3 were ineffective. The effect of PUFAs on oocyte maturation in vitro were corroborated with studies in vivo. Oocytes were obtained from females fed a commercial, PUFA-enriched diet (RD) and maturational behavior was compared with oocytes from females fed a natural diet (ND) with a higher EPA content and n-3:n-6 ratio. Although no significant difference was observed in the rate of spontaneous oocyte maturation, a higher percentage of GTH-induced maturation and lower percentage of atresia were observed in RD oocytes. Moreover, while basal PGE production from oocytes from both groups was the same, RD oocytes produced significantly higher levels of PGE in the presence of hCG. The results from this study provide evidence for the participation of AA metabolism in GTH-induced oocyte maturation, and suggest that other PUFAs and PGs may play important roles in the induction of maturation in a marine teleost.