Cellular organization and substructure measured using angle-resolved low-coherence interferometry
Cellular organization and substructure measured using angle-resolved low-coherence interferometry
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DOI：
10.1016/s0006-3495(02)75571-9
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发表时间：
2002-04-01
影响因子：
3.4
通讯作者：
Feld, MS
中科院分区：
文献类型：
--
作者：
Wax, A;Yang, CH;Feld, MS
我们使用角度分辨低相干干涉法测量单层HT29上皮细胞的组织和亚结构。这项新技术通过测量散射光来探测细胞结构，就像在流式细胞术中一样，但提供了一个优势，即可以原位检查结构，避免了破坏细胞单层的需要。我们通过将测量的光散射光谱与Mie理论的预测进行拟合来确定细胞核的尺寸分布。此外，我们通过检查单层内的空间相关性来获得关于细胞组织和亚结构的信息。一个值得注意的发现是，在小长度尺度上的空间相关性呈逆幂定律的形式，表明亚细胞结构的堆积具有分形性。我们还确定了与细胞大小相比规模较大的空间相关性，表明了单层内的覆盖顺序。
We measure the organization and substructure of HT29 epithelial cells in a monolayer using angle-resolved low-coherence interferometry. This new technique probes cellular structure by measuring scattered light, as in flow cytometry, but offers an advantage in that the structure can be examined in situ, avoiding the need to disrupt the cell monolayer. We determine the size distribution of the cell nuclei by fitting measured light-scattering spectra to the predictions of Mie theory. In addition, we obtain information about the cellular organization and substructure by examining the spatial correlations within the monolayer. A remarkable finding is that the spatial correlations over small length scales take the form of an inverse power law, indicating the fractal nature of the packing of the subcellular structures. We also identify spatial correlations on a scale large compared with the size of a cell, indicating an overlying order within the monolayer.