Involvement of Ethylene in the Accumulation of Esculeoside A during Fruit Ripening of Tomato (Solanum lycopersicum)
Involvement of Ethylene in the Accumulation of Esculeoside A during Fruit Ripening of Tomato (Solanum lycopersicum)
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DOI：
10.1021/jf8037902
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发表时间：
2009-04-22
影响因子：
6.1
通讯作者：
Shibata, Daisuke
中科院分区：
文献类型：
--
作者：
Iijima, Yoko;Fujiwara, Yukio;Shibata, Daisuke
番茄果实中糖生物碱的组成随成熟度的变化而变化。然而，目前还不清楚糖生物碱的积累是否受催熟激素乙烯的控制。本文报道了乙烯对番茄果实中糖生物碱积累的影响。考察了外源乙烯的影响。乙烯处理后，α -番茄碱含量降低，而皂苷A含量升高。接下来，我们分析了成熟突变体rin、nor和Nr的果实。在这些突变系的果实中，esculle苷A的积累水平下降，而α -番茄碱的积累水平高于野生型果实。这些结果表明，皂苷A的积累与乙烯的产生和感知有关。此外，成熟突变体果实中皮苷A生物合成的中间代谢物的积累曲线表明，从α -番茄素到皮苷A的糖基化过程依赖于乙烯。
The composition of glycoalkaloids in tomato fruit changes with ripening. However, it has not been clarified whether the accumulation of glycoalkaloids is controlled by the ripening-inducing phytohormone, ethylene. Here, we report the effect of ethylene on the accumulation of tomato fruit glycoalkaloids. We investigated the effect of exogenously applied ethylene. In response to ethylene treatment, the content of alpha-tomatine decreased, whereas the content of esculeoside A increased. Next, we analyzed the fruits of ripening mutants, rin, nor, and Nr. In fruits of these mutant lines, the level of accumulation of esculeoside A decreased, whereas alpha-tomatine accumulated to higher levels than in wild-type fruit. These results demonstrated that the esculeoside A accumulation was associated with production and perception of ethylene. Additionally, the accumulation profiles of the intermediate metabolites of esculeoside A biosynthesis in ripening mutant fruits suggest that a glycosylation step in the putative pathway from alpha-tomatine to esculeoside A depends on ethylene.