PHLOEM LOADING IN VICIA-FABA LEAVES - EFFECT OF N-ETHYLMALEIMIDE AND PARACHLOROMERCURIBENZENESULFONIC ACID ON H+ EXTRUSION, K+ AND SUCROSE UPTAKE
PHLOEM LOADING IN VICIA-FABA LEAVES - EFFECT OF N-ETHYLMALEIMIDE AND PARACHLOROMERCURIBENZENESULFONIC ACID ON H+ EXTRUSION, K+ AND SUCROSE UPTAKE
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DOI：
10.1007/bf00380875
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发表时间：
1980-01-01
期刊：
影响因子：
4.3
通讯作者：
BONNEMAIN, JL
中科院分区：
文献类型：
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作者：
DELROT, S;DESPEGHEL, JP;BONNEMAIN, JL
研究了穿透性（NEM = n-乙基马来酰亚胺）和非穿透性（PCMBS =对氯苯磺酸）巯基特异性试剂对蚕豆叶片质子挤出、86Rb和[U-14C]蔗糖吸收的影响。0.1 mM NEM强烈或完全抑制质子挤压。在实验条件下，PCMBS （1 mM）对[14C]蔗糖的摄取有较强的抑制作用，但对质子挤压和86Rb的摄取没有抑制作用。韧皮部负载对PCMBS的敏感性可能是糖载体堵塞的结果，而不是质子泵抑制的结果。
The effects of a penetrating (NEM = N-ethylmaleimide) and a non-penetrating (PCMBS = p-chloromercuribenzenesulfonic acid) sulfhydryl-specific reagent on proton extrusion, 86Rb and [U-14C]sucrose uptake by V. faba leaves were studied. Proton extrusion was strongly or completely inhibited by 0.1 mM NEM. 86Rb and [U-14C]sucrose uptake were markedly reduced by NEM concentrations equal to or higher than 0.5 mM. Under experimental conditions, PCMBS (1 mM) exerted a strong inhibition on [14C]sucrose uptake but did not inhibit proton extrusion and 86Rb uptake. The sensitivity of phloem loading to PCMBS may be a consequence of sugar-carrier blockage and not of inhibition of the proton pump.