Repair and recombination of nonreplicating UV-irradiated phage DNA in E. coli II. Stimulation of RecF-dependent recombination by excision repair of cyclobutane pyrimidine dimers and of other photoproducts.
Repair and recombination of nonreplicating UV-irradiated phage DNA in E. coli II. Stimulation of RecF-dependent recombination by excision repair of cyclobutane pyrimidine dimers and of other photoproducts.
复制标题
大肠杆菌 II 中非复制型紫外线照射噬菌体 DNA 的修复和重组。
DOI：
10.1007/bf00331329
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Hays,JB
中科院分区：
文献类型：
--
作者：
Smith,TA;Hays,JB
紫外线照射的非复制λ噬菌体DNA的重组的三个方面进行了讨论：负责的光产物，UvrABC介导的切除修复的作用，和RecF功能的依赖性。环丁烷嘧啶二聚体似乎负责一些重组，因为光复活减少的频率254 nm刺激的重组，因为光敏313 nm照射刺激的重组。其他photoproducts似乎recombinogenic以及，因为高通量的254 nm照射刺激重组相当多，每环丁烷二聚体诱导，比光敏313 nm照射，因为光复活没有消除254 nm刺激重组。对于这两种治疗，大部分，但不是全部，重组是紫外线ABC依赖。主要依赖于RecF，但不受recB recCorrectE突变的影响
Three aspects of recombination of UV-irradiated nonreplicating lambda phage DNA were addressed: the photoproduct(s) responsible, the role of UvrABC-mediated excision repair, and the dependence on RecF function.Cyclobutane pyrimidine dimers appeared responsible for some recombination because photoreactivation reduced the frequency of 254-nm-stimulated recombination and because photosensitized 313-nm irradiation stimulated recombination. Other photoproducts seemed recombinogenic as well, because high fluences of 254-nm irradiation stimulated recombination considerably more, per cyclobutane dimer induced, than photosensitized 313-nm irradiation, and because photoreactivation did not eliminate 254-nm stimulated recombination. For both treatments, much, but not all, of the recombination was UvrABC-dependent. Recombination was mostly RecF-dependent, but was not affected byrecB recCorrecEmutations