FUNCTIONAL-ROLE OF CD26 ON HUMAN B-LYMPHOCYTES
FUNCTIONAL-ROLE OF CD26 ON HUMAN B-LYMPHOCYTES
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DOI：
10.1016/0165-2478(94)00230-o
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发表时间：
1995-02-01
影响因子：
4.4
通讯作者：
ANSORGE, S
中科院分区：
文献类型：
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作者：
BUHLING, F;JUNKER, U;ANSORGE, S
CD 26是T细胞和自然杀伤（NK）细胞上的众所周知的活化标志物[1]。它与外肽酶二肽基肽酶IV（DP IV）相同。关于B细胞上CD 26的表达一直存在争议[2，3]。我们已经研究了这种酶在健康供体和CVID患者外周血的B细胞、Daudi Burkitt细胞系和EBV转化的B细胞系Jojo和Laz 509上的表达。使用抗CD 26单克隆抗体并借助特异性酶底物检测DP IV。进一步研究了特异性合成DP IV抑制剂对纯化的B细胞的有丝分裂活化和细胞系DNA合成的影响。我们可以证明，在两组中，0-5%的新鲜分离的CD 20阳性B细胞表达CD 26抗原。在用美洲商陆有丝分裂原或金黄色葡萄球菌蛋白刺激后，CD 26阳性细胞的分数分别增加到51%和36%。有趣的是，CVID患者的B细胞上CD 26表达的诱导以与健康供体的B细胞相似的方式发生。用高度特异性的竞争性DP IV抑制剂处理外周血B细胞和B细胞系导致DNA合成以剂量依赖性方式显著抑制。这些数据表明，CD 26不仅可以被认为是T细胞和NK细胞的活化标志物，而且可以被认为是B细胞的主要群体的活化标志物，这表明CD 26参与B细胞活化。
CD26 is a well-known activation marker on T cells and natural killer (NK) cells [1]. It is identical with the ectopeptidase dipeptidyl peptidase IV (DP IV). The expression of CD26 on B cells has been discussed controversially [2,3]. We have studied the expression of this enzyme on B cells from the peripheral blood of healthy donors and of CVID patients, on cells of the Daudi Burkitt line and the EBV-transformed B-cell lines Jojo and Laz509. DP IV was detected by using anti-CD26 monoclonal antibodies and with help of specific enzyme substrates. Further the influence of specific synthetic DP IV inhibitors on mitogenic activation of purified B cells and DNA synthesis of cell lines was studied. We could show that in both groups 0-5% of freshly isolated CD20-positive B cells do express the CD26 antigen. After stimulation with pokeweed mitogen or St. aureus protein, the fraction of CD26-positive cells was enhanced up to 51% and 36%, respectively. Interestingly, induction of CD26 expression on B cells from CVID patients occurs in a manner similar to the B cells from healthy donors. Treatment of peripheral blood B cells and B-cell lines with highly specific competitive DP IV inhibitors leads to a significant inhibition of DNA synthesis in a dose-dependent manner. These data show that CD26 can be considered to be an activation marker not only of T- and NK cells but also of a main population of B cells, suggesting an involvement of CD26 in B-cell activation.