Ancient genome-wide admixture extends beyond the current hybrid zone between Macaca fascicularis and M-mulatta
Ancient genome-wide admixture extends beyond the current hybrid zone between Macaca fascicularis and M-mulatta
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DOI：
10.1111/j.1365-294x.2010.04687.x
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发表时间：
2010-07-01
影响因子：
4.9
通讯作者：
Terao, Keiji
中科院分区：
文献类型：
--
作者：
Osada, Naoki;Uno, Yasuhiro;Terao, Keiji
食蟹猴和猕猴是两种最常用的实验室猕猴，但它们在全基因组水平上的遗传差异仍不清楚。我们分析了从M. fascicularis样品来自三个不同的地理起源和M.来自缅甸的穆拉塔样本。M.食蟹猴的核苷酸多样性很高，是人类的四到五倍，并且在马来西亚猕猴和菲律宾猕猴之间有很强的地理种群结构。对M. fascicularis和M. mulatta显示了基因交换的足迹，不仅在他们目前的杂交区，而且在更广泛的范围内超过100万年。然而，遗传混合可能不是基因组中的随机事件。而随机选择的基因和基因间区域具有相同的物种之间的进化动力学，一些细胞色素氧化酶P450（CYP450）基因（主要的化学代谢基因和潜在的目标基因的本地适应）有一个显着较大的物种比其他基因的分歧。通过调查超过100猕猴的CYP3A5基因序列，我们确定了三个非同义的单核苷酸多态性是高度分化的猕猴之间。基因组中物种分歧的马赛克模式可能是生态适应下遗传分化的结果，也可能是近保护下新生物种基因组的一个显着特征。
Macaca fascicularis and Macaca mulatta are two of the most commonly used laboratory macaques, yet their genetic differences at a genome-wide level remain unclear. We analysed the multilocus DNA sequence data of 54 autosomal loci obtained from M. fascicularis samples from three different geographic origins and M. mulatta samples of Burmese origin. M. fascicularis shows high nucleotide diversity, four to five times higher than humans, and a strong geographic population structure between Indonesian-Malaysian and Philippine macaques. The pattern of divergence and polymorphism between M. fascicularis and M. mulatta shows a footprint of genetic exchange not only within their current hybrid zone but also across a wider range for more than 1 million years. However, genetic admixture may not be a random event in the genome. Whereas randomly selected genic and intergenic regions have the same evolutionary dynamics between the species, some cytochrome oxidase P450 (CYP) genes (major chemical metabolizing genes and potential target genes for local adaptation) have a significantly larger species divergence than other genes. By surveying CYP3A5 gene sequences of more than a hundred macaques, we identified three nonsynonymous single nucleotide polymorphisms that were highly differentiated between the macaques. The mosaic pattern of species divergence in the genomes may be a consequence of genetic differentiation under ecological adaptation and may be a salient feature in the genomes of nascent species under parapatry.