Structure and Expression of a Gene for the Large Subunit of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase from Pine
Structure and Expression of a Gene for the Large Subunit of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase from Pine
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松树核酮糖-1,5-二磷酸羧化酶/加氧酶大亚基基因的结构和表达
DOI：
10.1093/oxfordjournals.pcp.a078074
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发表时间：
1991
影响因子：
4.9
通讯作者：
K. Shinohara
中科院分区：
文献类型：
--
作者：
Y. Mukai;N. Yamamoto;K. Odani;K. Shinohara
测定了松核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基（LSU）基因的核苷酸序列。LSU的pine基因（rbcL）全长1,425 bp，编码475个氨基酸。其核苷酸序列和推导的氨基酸序列与被子植物的相应序列具有很高的同源性。TherbcL转录起始于起始密码子上游约120个核苷酸，终止于终止密码子下游约120个核苷酸。该启动子序列与被子植物的启动子序列高度同源。在对应于转录本3 '端的区域中发现两个反向重复序列。然而，反向重复序列中的核苷酸序列与被子植物中的此类序列非常不同。由于积累的LSU，以及叶绿素，已被观察到在黑暗中生长的松树幼苗，我们研究了pinerbcLunder的表达-各种条件。在松树种子萌发过程中，即使在黑暗中，therbcL转录水平也会增加。基因剂量也以不依赖于光的方式增加。此外，似乎成熟的转录物储存在干胚中，并且转录发生在吸胀的三天内。结果表明，rbcL在松树种子萌发过程中持续表达。
The nucleotide sequence of the gene for the large subunit (LSU) of ribulose-l,5-bisphosphate carboxylase/oxygenase (RuBisCO) in pine(Pinus thunbergii)was determined. The pine gene for LSU(rbcL)contained 1,425 bp which encoded 475 amino acids. The nucleotide sequence and the deduced amino acid sequence each showed a very high degree of homology to the corresponding sequences from angiosperms. TherbcLtranscript started from about 120 nucleotides upstream from the initiation codon and terminated about 120 nucleotides downstream from the termination codon. The promoter sequence was highly homologous to those of angiosperms. Two inverted repeats were found in the region that corresponded to the 3'-terminus of the transcript. However, the nucleotide sequence in the inverted repeats was very different from such sequences in angiosperms. Since the accumulation of LSU, as well as that of chlorophylls, has been observed in dark-grown pine seedlings, we studied the expression of the pinerbcLunder-various conditions. Levels of therbcLtranscript increased even in the dark during the germination of pine seeds. The gene dosage also increased in a light-independent manner. In addition, it appeared that the mature transcript was stored in dry embryos and that transcription occurred within three days of imbibition. The results suggest thatrbcLis expressed continuously during the germination of pine seeds.