Neural cell adhesion molecule (N-CAM) is elevated in adult avian slow muscle fibers with multiple terminals.
Neural cell adhesion molecule (N-CAM) is elevated in adult avian slow muscle fibers with multiple terminals.
复制标题
成年禽多末端慢肌纤维中的神经细胞粘附分子 (N-CAM) 升高。
DOI：
10.1073/pnas.86.16.6403
复制
发表时间：
1989
影响因子：
11.1
通讯作者：
Nottebohm,F
中科院分区：
文献类型：
--
作者：
Bleisch,W;Scharff,C;Nottebohm,F
许多成年鸟类肌肉含有两种类型的肌纤维：在单焦点末端接受神经支配的肌纤维和具有多个末端的肌纤维。鸟类的发声器官——鸣管的肌肉就是这样的混合肌肉。为了研究纤维类型和神经支配的异质性，我们结合免疫细胞化学对肌肉纤维进行分类，并使用可视化神经肌肉接头的技术。一种单克隆抗体 S58，针对一类慢速肌球蛋白，仅标记具有多个末端的纤维。我们还检查了神经细胞粘附分子（N-CAM）的免疫反应性分布，该分子被认为在肌肉神经支配和神经肌肉接头形成中发挥作用。 S58 阳性纤维的 N-CAM 染色升高，表明纤维的多重神经支配与纤维高水平 N-CAM 免疫反应性的表达相关。大多数（也许是全部）具有多个末端的纤维也含有丰富的 N-CAM 免疫反应性。这表明 N-CAM 可能在成人受神经支配的肌肉的多末端神经支配的维持中发挥作用。
Many adult avian muscles contain two types of muscle fiber: those that receive innervation at single focal terminals and those with multiple terminals. The muscles of the syrinx, the vocal organ of birds, are such mixed muscles. To study this heterogeneity of fiber type and innervation, we combined immunocytochemistry to classify muscle fibers with techniques to visualize neuromuscular junctions. One monoclonal antibody, S58, directed against a slow class of myosin, labels only fibers that have multiple terminals. We also examined the distribution of immunoreactivity for neural cell adhesion molecule (N-CAM), which has been suggested to play a role in innervation of muscle and formation of neuromuscular junctions. S58-positive fibers have elevated N-CAM staining, indicating that multiple innervation of a fiber is correlated with the fiber's expression of high levels of N-CAM immunoreactivity. Most, and perhaps all, fibers that have multiple terminals also contain abundant N-CAM immunoreactivity. This suggests that N-CAM may play a role in the maintenance of multiterminal innervation in adult innervated muscle.