Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens.
Identification of the receptors for prolactin-releasing peptide (PrRP) and Carassius RFamide peptide (C-RFa) in chickens.
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DOI：
10.1210/en.2011-1719
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发表时间：
2012-02
期刊：
影响因子：
4.8
通讯作者：
Yajun Wang;C. Wang;Yuting Wu;Guian Huang;Juan Li;F. C. Leung
中科院分区：
文献类型：
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作者：
Yajun Wang;C. Wang;Yuting Wu;Guian Huang;Juan Li;F. C. Leung
据报道，催乳素释放肽（PrRP）及其结构相关肽鲫鱼精氨酸-苯丙氨酸酰胺肽（C-RFa）在调节脊椎动物的食物摄入和垂体功能方面发挥着相似的作用。然而，在包括鸟类在内的非哺乳动物脊椎动物中，PrRP 和 C-RFa 受体的身份、功能和表达仍然很大程度上未知。本研究通过RT-PCR从鸡脑中克隆了3个与哺乳动物PrRP受体（PrRPR）同源的受体，分别命名为cPrRPR1、cPrRPR2和cC-RFaR。使用pGL3-NFAT-RE-荧光素酶报告系统，我们证明了中国仓鼠卵巢细胞中表达的cPrRPR1和cPrRPR2可以被cPrRP20和cC-RFa20有效激活，而cC-RFaR只能被cC-RFa20（EC50，0.11 nM）有效激活，这表明cPrRPR1和cPrRPR1和cPrRPR2可以被cC-RFa20激活。 cPrRPR2 可作为 PrRP 和 C-RFa 的共同受体，而 cC-RFaR 是 C-RFa 的特异性受体。使用 pGL3-CRE-荧光素酶报告系统，中国仓鼠卵巢细胞中表达的 cPrRPR1、cPrRPR2 和 cC-RFaR 在 cC-RFa20（100 nM）处理后也能激活细胞内蛋白激酶 A 信号通路。此外，RT-PCR检测显示cPrRPR1、cPrRPR2和cC-RFaR在大多数成年鸡组织中广泛表达，包括大脑的各个区域。这些发现，加上鸡、爪蟾和斑马鱼中不同基因编码的PrRP和C-RFa的证据，以及鸡组织中PrRP和C-RFa基因的差异表达，强烈表明PrRP和C-RFa可能在包括鸡在内的非哺乳动物脊椎动物中发挥相似但独特的作用，并且它们的作用是由共同受体或特定C-RFa受体介导的。
Prolactin-releasing peptide (PrRP) and its structurally related peptide, Carassius Arg-Phe-amide peptide (C-RFa), have been reported to play similar roles in regulating food intake and pituitary functions in vertebrates. However, the identity, functionality, and expression of the receptor(s) for PrRP and C-RFa remain largely unknown in nonmammalian vertebrates, including birds. In this study, three receptors homologous to mammalian PrRP receptor (PrRPR), named cPrRPR1, cPrRPR2, and cC-RFaR, respectively, were cloned from chicken brain by RT-PCR. Using a pGL3-NFAT-RE-luciferase reporter system, we demonstrated that cPrRPR1 and cPrRPR2 expressed in Chinese hamster ovarian cells could be activated by cPrRP₂₀ and cC-RFa₂₀ potently, whereas cC-RFaR could only be activated effectively by cC-RFa₂₀ (EC₅₀, 0.11 nM), indicating that cPrRPR1 and cPrRPR2 can function as common receptors for PrRP and C-RFa, whereas cC-RFaR is a receptor specific to C-RFa. Using a pGL3-CRE-luciferase reporter system, cPrRPR1, cPrRPR2, and cC-RFaR expressed in Chinese hamster ovarian cells were also shown to activate intracellular protein kinase A signaling pathway upon cC-RFa₂₀ treatment (100 nM). Moreover, RT-PCR assay revealed that cPrRPR1, cPrRPR2, and cC-RFaR were widely expressed in most adult chicken tissues examined, including various regions of brain. These findings, together with evidence of PrRP and C-RFa encoded by separate genes in chicken, Xenopus, and zebrafish, and the differential expression of PrRP and C-RFa genes in chicken tissues, strongly suggest that PrRP and C-RFa may play similar yet distinctive roles in nonmammalian vertebrates, including chicken, and their actions are mediated by common receptor(s) or a specific C-RFa receptor.