Blood and immune cell engineering: Cytoskeletal contractility and nuclear rheology impact cell lineage and localization: Biophysical regulation of hematopoietic differentiation and trafficking.
Blood and immune cell engineering: Cytoskeletal contractility and nuclear rheology impact cell lineage and localization: Biophysical regulation of hematopoietic differentiation and trafficking.
复制标题
血液和免疫细胞工程：细胞骨架收缩力和核流变学影响细胞谱系和定位：造血分化和运输的生物物理调节。
DOI：
10.1002/bies.201400166
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Discher DE
中科院分区：
文献类型：
--
作者：
Shin JW;Discher DE
Clinical success with human hematopoietic stem cell (HSC) transplantation establishes a paradigm for regenerative therapies with other types of stem cells. However, it remains challenging to repair or replace tissues after engineering stem cells in vitro. Recent studies suggest that stem cells sense physical features of their niches. Here we review biophysical contributions to lineage decisions, maturation, and trafficking in the hematopoietic system. Polarized cellular contractility and nuclear rheology are separately shown to be functional markers of a hematopoietic hierarchy that predict the ability of a lineage to traffic in and out of the bone marrow niche. These biophysical determinants are ultimately regulated by a set of structural molecules, including cytoplasmic myosin-II and nuclear lamins, which themselves are modulated by a diverse range of transcriptional and post-translational mechanisms. Small molecules that target these mechanobiological circuits, along with novel bioengineering methods, could prove broadly useful in programming stem cells for therapy.
