bZIP transcription factor CgAP1 is essential for oxidative stress tolerance and full virulence of the poplar anthracnose fungus Colletotrichum gloeosporioides
bZIP transcription factor CgAP1 is essential for oxidative stress tolerance and full virulence of the poplar anthracnose fungus Colletotrichum gloeosporioides
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bZIP 转录因子 CgAP1 对于杨树炭疽真菌胶孢炭疽菌的氧化应激耐受性和完全毒力至关重要
DOI：
10.1016/j.fgb.2016.08.006
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发表时间：
2016-10-01
影响因子：
3
通讯作者：
Tian, Chengming
中科院分区：
文献类型：
--
作者：
Sun, Yingjiao;Wang, Yonglin;Tian, Chengming
酵母AP1转录因子是氧化应激反应的调节因子。在这里，我们报道了杨树炭疽菌球孢炭疽菌中YAP1的同源基因CgAP1的鉴定和鉴定。CgAP1的表达受到活性氧的高度诱导。与野生型菌株相比，CgAP1缺失突变体表现出对氧化胁迫的敏感性增强，其杨树叶片毒力明显降低。然而，突变体在气生菌丝生长、分生孢子产生和附着体形成方面没有明显的缺陷。经TBH(叔丁基氢过氧化氢)处理后，CgAP1：：EGFP融合蛋白定位于细胞核，提示CgAP1在球孢子虫中具有氧化还原感受器的作用。此外，CgAP1在生物营养生长的早期阶段阻止了ROS的积累。CgAP1也是参与抗氧化反应的几个ROS相关基因(即Glr1、Hyr1和Cyt1)的正调控因子。这些结果突出了CgAP1转录因子在氧化应激反应中的关键调控作用，并为ROS解毒在胶孢子虫毒力中的作用提供了深入的认识。(C)2016 Elsevier Inc.保留所有权利。
Yeast AP1 transcription factor is a regulator of oxidative stress response. Here, we report the identification and characterization of CgAP1, an ortholog of YAP1 in poplar anthracnose fungus Colletotrichum gloeosporioides. The expression of CgAP1 was highly induced by reactive oxygen species. CgAP1 deletion mutants displayed enhanced sensitivity to oxidative stress compared with the wild-type strain, and their poplar leaf virulence was obviously reduced. However, the mutants exhibited no obvious defects in aerial hyphal growth, conidia production, and appressoria formation. CgAP1::eGFP fusion protein localized to the nucleus after TBH (tert-Butyl hydroperoxide) treatment, suggesting that CgAP1 functions as a redox sensor in C. gloeosporioides. In addition, CgAP1 prevented the accumulation of ROS during early stages of biotrophic growth. CgAP1 also acted as a positive regulator of several ROS-related genes (i.e., Glr1, Hyr1, and Cyt1) involved in the antioxidative response. These results highlight the key regulatory role of CgAP1 transcription factor in oxidative stress response and provide insights into the function of ROS detoxification in virulence of C gloeosporioides. (C) 2016 Elsevier Inc. All rights reserved.