Therapeutic effects and molecular mechanisms of anti-fibrosis herbs and selenium on rats with hepatic fibrosis
Therapeutic effects and molecular mechanisms of anti-fibrosis herbs and selenium on rats with hepatic fibrosis
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DOI：
10.3748/wjg.v10.i5.703
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发表时间：
2004-03-01
影响因子：
4.3
通讯作者：
Xiao, Yong-Hong
中科院分区：
文献类型：
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作者：
He, Yu-Tong;Liu, Dian-Wu;Xiao, Yong-Hong
目的：研究抗纤维化中药和硒对四氯化碳（CCl4）致大鼠肝纤维化的治疗作用及其分子机制。方法：将53只Wistar大鼠随机分为：正常对照组、模型对照组、秋水仙碱组、抗纤维化中药组（AF组）和抗纤维化中药加硒组（AS组）。后4组在实验开始时给予CCl4诱导肝纤维化。然后用秋水仙碱、抗纤维化中药和硒治疗。正常对照组和模型对照组同时给予生理盐水治疗。第6周末，处死各组大鼠。采集血液和组织标本。测定血清各项指标（ALT、AST、HA、LN），并对组织病理变化进行分级。流式细胞术检测淋巴细胞CD4、CD8。免疫组化检测tgf - β(1)和nf - κ B的表达，半定量RT-PCR检测tgf - β (1) mRNA的表达。结果：组织学分级显示，AS组和AF组肝纤维化程度明显小于秋水仙碱组和模型对照组。AF组和AS组血清ALT、AST、HA和LN含量显著低于秋水仙碱组（AF组、AS组和秋水仙碱组ALT分别为65.8 +/- 26.5、67.3 +/- 18.4和96.2 +/- 20.9，AST分别为150.8 +/- 34.0、154.6 +/- 27.3和215.8 +/- 24.6，HA分别为228 +/- 83、216 +/- 58和416 +/- 135，LN分别为85.9 +/- 15.0、80.6 +/- 18.6和106.3 +/- 14.2）
AIM: To study the therapeutic effects of anti-fibrosis herbs and selenium on hepatic fibrosis induced by carbon tetrachloride (CCl4) in rats and the underlining molecular mechanisms.METHODS: Fifty-three Wistar rats were randomly divided into: normal control group, model control group, colchicine group, anti-fibrosis herbs group (AF group) and anti-fibrosis herbs plus selenium group (AS group). The last four groups were administered with CCl4 at the beginning of experiment to induce hepatic fibrosis. Then colchicine, anti-fibrosis herbs and selenium were used to treat them. The normal control group and the model control group were given normal saline at the same time. At the end of the 6(th) week, rats in each group were sacrificed. Blood and tissue specimens were taken. Serum indicators (ALT, AST, HA, LN) were determined and histopathological changes were graded. Lymphocyte CD4 and CD8 were examined by flow cytometry. Expression of TGF-beta(1) and NF-kappa B was detected by immunohistochemistry and expression of TGF-beta(1) mRNA was detected by semi-quantified RT-PCR.RESULTS: Histological grading showed much a smaller degree of hepatic fibrogenesis in AS group and AF group than that in colchicine group and model control group. The serum content of ALT, AST, HA and LN in AF group and AS group were significantly lower than that in colchicine group (ALT: 65.8 +/- 26.5, 67.3 +/- 18.4 and 96.2 +/- 20.9 in AF, AS and colchicine groups respectively; AST: 150.8 +/- 34.0, 154.6 +/- 27.3 and 215.8 +/- 24.6 respectively; HA: 228 +/- 83, 216 +/- 58 and 416 +/- 135 respectively; LN: 85.9 +/- 15.0, 80.6 +/- 18.6 and 106.3 +/- 14.2 respectively) (P