Thermostable DNA polymerases can perform translesion synthesis using 8-oxoguanine and tetrahydrofuran-containing DNA templates
Thermostable DNA polymerases can perform translesion synthesis using 8-oxoguanine and tetrahydrofuran-containing DNA templates
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DOI：
10.1016/j.bbapap.2005.11.001
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发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Lavrik, OI
中科院分区：
文献类型：
--
作者：
Belousova, EA;Rechkunova, NI;Lavrik, OI
研究了利用合成的脱碱基位点、四氢呋喃（THF）和含8-氧代鸟嘌呤的DNA模板的热稳定DNA聚合酶Taq、Tte和Tte-seq的跨损伤合成（TLS）能力。使用人DNA聚合酶β的测量用作“阳性对照”。观察到热稳定DNA聚合酶在两种底物上以不同的特异性进行TLS。在含有THF的模板下，dGMP优先被所有的DNA聚合酶插入。在Mn（II）作为辅因子的存在下，所有的聚合酶掺入dCMP相反的8-oxoguanine，而在Mg（II）离子的存在下，dAMP被纳入。结果发现，没有一个嗜热DNA聚合酶利用dTTP与8-氧代鸟嘌呤或含THF的模板。在所有情况下，含有THF作为损伤的DNA双链体加工成全长的效率低于含有8-氧代鸟嘌呤的DNA双链体。(c)2005 Elsevier B. V.保留所有权利。
The translesion synthesis (TLS) capacity of the thermostable DNA polymerases Taq, Tte and Tte-seq utilizing a synthetic abasic site, tetrahydrofuran (THF), and an 8-oxoguanine-containing DNA template was investigated. Measurements with human DNA polymerase beta were used as a "positive control". Thermostable DNA polymerases were observed to perform TLS with different specificities on both substrates. With a THF-containing template, dGMP was preferentially inserted by all the DNA polymerases. In the presence of Mn(II) as a cofactor, all the polymerases incorporated dCMP opposite 8-oxoguanine whereas, in the presence of Mg(II) ions, dAMP was incorporated. It was found that none of the thermophilic DNA polymerases utilized dTTP with either an 8-oxoguanine or a THF-containing template. In all cases, DNA duplex containing THF as damage was processed to full length less effectively than DNA duplex containing 8-oxoguanine. (c) 2005 Elsevier B.V. All rights reserved.