COLONIZING POPULATIONS OF CANDIDA-ALBICANS ARE CLONAL IN ORIGIN BUT UNDERGO MICROEVOLUTION THROUGH C1 FRAGMENT REORGANIZATION AS DEMONSTRATED BY DNA-FINGERPRINTING AND C1 SEQUENCING
COLONIZING POPULATIONS OF CANDIDA-ALBICANS ARE CLONAL IN ORIGIN BUT UNDERGO MICROEVOLUTION THROUGH C1 FRAGMENT REORGANIZATION AS DEMONSTRATED BY DNA-FINGERPRINTING AND C1 SEQUENCING
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DOI：
10.1128/jcm.33.6.1501-1509.1995
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发表时间：
1995-06-01
影响因子：
9.4
通讯作者：
SOLL, DR
中科院分区：
文献类型：
--
作者：
LOCKHART, SR;FRITCH, JJ;SOLL, DR
对9个白色念珠菌共生和感染群体的遗传同源性进行了鉴定，方法是先用CA3探针，然后用CA3探针的0.98kbC_1片段，对每个群体的多个分离株进行Southern印迹杂交。每个种群的分离物高度相关，证明了每个种群的克隆起源，但每个种群都含有微小的变异，表明了微进化。每种情况下的变异都局限于与0.98kb的c1片段杂交的CA3指纹图谱的条带，因此对c1片段进行了测序，并证明含有RPS重复元件。因此，这些结果表明，在非免疫抑制患者中，大多数定植的白色念珠菌种群是克隆的，通过C1杂交可以在每个定植种群中检测到微进化，并且C1包含重复的RPS元件。
The genetic homogeneity of nine commensal and infecting populations of Candida albicans has been assessed by fingerprinting multiple isolates from each population by Southern blot hybridization first with the Ca3 probe and then with the 0.98-kb C1 fragment of the Ca3 probe. The isolates from each population were highly related, demonstrating the clonal origin of each population, but each population contained minor variants, demonstrating microevolution. Variation in each case was limited to bands of the Ca3 fingerprint pattern which hybridized with the 0,98-kb C1 fragment, The C1 fragment was therefore sequenced and demonstrated to contain an RPS repetitive element. The C1 fragment also contained part or all of a true end of the RPS element, These results, therefore, demonstrate that most colonizing C. albicans populations in nonimmunosuppressed patients are clonal, that microevolution can be detected in every colonizing population by C1 hybridization, and that C1 contains the repeat RPS element.