Intact metabolite profiling of mouse brain by probe electrospray ionization/triple quadrupole tandem mass spectrometry (PESI/MS/MS) and its potential use for local distribution analysis of the brain
Intact metabolite profiling of mouse brain by probe electrospray ionization/triple quadrupole tandem mass spectrometry (PESI/MS/MS) and its potential use for local distribution analysis of the brain
复制标题
DOI：
10.1016/j.aca.2017.06.047
复制
发表时间：
2017-08-29
影响因子：
6.2
通讯作者：
Ishikawa, Tetsuya
中科院分区：
文献类型：
--
作者：
Hayashi, Yumi;Zaitsu, Kei;Ishikawa, Tetsuya
探针电喷雾电离（PESI），这是一种环境电离技术，使我们能够分析完整的内源性代谢物，而无需样品制备。在这项研究中，我们应用了新开发的PESI耦合串联质谱（PESI/MS/MS）的方法来分析代谢物在小鼠脑中，其中其脂质组成往往干扰MS为基础的代谢组学分析。结果，PESI/MS/MS直接检测到小鼠额叶皮层中的25种代谢物，并清楚地区分了能量代谢障碍小鼠模型和对照小鼠的代谢谱。PESI/MS/MS还允许我们对每只小鼠（n = 5）的海马和额叶皮层进行局部分布分析，区分它们的细微代谢差异。这些结果显示PESI/MS/MS用于小鼠脑的直接代谢组分析的高潜力。(C)2017爱思唯尔B. V.保留所有权利。
Probe electrospray ionization (PESI), which is an ambient ionization technique, enables us to analyze intact endogenous metabolites without sample preparation. In this study, we applied the newly developed method of PESI coupled to tandem mass spectrometry (PESI/MS/MS) to analyze metabolites in mouse brain, where its lipid composition often interfere with MS-based metabolome analysis. As a result, PESI/MS/MS directly detected 25 metabolites in a mouse frontal cortex, and clearly discriminated the metabolic profiles of mice model with energy metabolism disruption from control mice. PESI/MS/MS also allowed us to perform local distribution analysis of the hippocampus as well as the frontal cortex in each mouse (n = 5), discriminating their subtle metabolic differences. These results showed high potential of PESI/MS/MS for direct metabolome profiling of mouse brain. (C) 2017 Elsevier B.V. All rights reserved.