Activation of Ca2+/calmodulin-dependent protein kinase II is involved in hyperosmotic induction of the human taurine transporter
Activation of Ca2+/calmodulin-dependent protein kinase II is involved in hyperosmotic induction of the human taurine transporter
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DOI：
10.1016/j.febslet.2004.05.062
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发表时间：
2004-07-02
期刊：
影响因子：
3.5
通讯作者：
Shimizu, M
中科院分区：
文献类型：
--
作者：
Satsu, H;Manabe, M;Shimizu, M
我们通过使用各种细胞内信号分子的特异性抑制剂，研究了参与人牛磺酸转运蛋白（TAUT）高渗调节的信号通路。其中，钙/钙调素依赖性蛋白激酶II（Ca 2 +/CaM激酶II）的特异性抑制剂完全抑制TAUT的高渗调节。钙调素拮抗剂和钙螯合剂也可显著抑制TAUT的神经元敏感性上调。特异性Ca 2 +/CaM激酶II抑制剂可抑制高渗引起的TAUT mRNA表达水平的升高。激活形式的Ca 2 +/CaM激酶II蛋白只能在高渗条件下的Caco-2细胞中检测到。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
We investigated the signaling pathways participating in hyperosmotic regulation of the human taurine transporter (TAUT) by using specific inhibitors of various intracellular signaling molecules. Among them, the specific inhibitor of calcium/calmodulin-dependent protein kinase II (Ca2+/CaM kinase II) completely repressed the hyperosmotic regulation of TAUT. The osmosensitive upregulation of TAUT was also significantly inhibited by calmodulin antagonists and calcium-chelators. The increased expression level of TAUT mRNA by hypertonicity was repressed by the specific Ca2+/CaM kinase II inhibitor. The activated form of Ca2+/CaM kinase II protein could only be detected in Caco-2 cells under hypertonic conditions. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.