Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans)
Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans)
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DOI：
10.1007/s004380050800
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发表时间：
1998-08-01
期刊：
影响因子：
--
通讯作者：
Waugh, R
中科院分区：
文献类型：
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作者：
Meyer, RC;Milbourne, D;Waugh, R
我们构建了一张整合了单、双和双单AFLP标记的四倍体马铃薯部分连锁图谱。该图谱由231个母本标记和106个父本标记组成，总图谱长度分别为990.9cM和484.6cM。两个累积图谱长度中较长的代表基因组的约25%覆盖。在四倍体中，亲本克隆之间的许多多态性被“剂量”掩盖，这显著减少了群体中可以评分的个体标记的数量。因此，使用AFLP的主要优点-其高多重比率-被降低到使用替代的多等位基因标记类型将显著更有效的程度。分离数据和图谱信息已被用于晚疫病抗性的QTL分析，并已确定在第VIII染色体近端的多等位基因位点，这对抗性的表达有显着贡献。没有晚疫病抗性基因或QTL先前已被定位到这个位置。
We have constructed a partial linkage map in tetraploid potato which integrates simplex, duplex and double-simplex AFLP markers. The map consists of 231 maternal and 106 paternal markers with total map lengths of 990.9 cM and 484.6 cM. The longer of the two cumulative map lengths represents approximately 25% coverage of the genome. In tetraploids, much of the polymorphism between parental clones is masked by 'dosage' which significantly reduces the number of individual markers that can be scored in a population. Consequently, the major advantage of using AFLPs - their high multiplex ratio - is reduced to the point where the use of alternative multi-allelic marker types would be significantly more efficient. The segregation data and map information have been used in a QTL analysis of late blight resistance, and a multi-allelic locus at the proximal end of chromosome VIII has been identified which contributes significantly to the expression of resistance. No late blight resistance genes or QTLs have previously been mapped to this location.