Expression of pentraxin 3 (PTX3) in human atherosclerotic lesions
Expression of pentraxin 3 (PTX3) in human atherosclerotic lesions
复制标题
DOI：
10.1002/path.2314
复制
发表时间：
2008-05-01
影响因子：
7.3
通讯作者：
Naito, M.
中科院分区：
文献类型：
--
作者：
Savchenko, A. S.;Imamura, M.;Naito, M.
五肽3(PTX3)和C反应蛋白(CRP)是五肽超家族的成员。PTX3的表达是由炎症信号诱导的，在炎症部位由几种类型的细胞产生，主要是单核/巨噬细胞、树突状细胞(DC)、内皮细胞、平滑肌细胞(SMCs)和成纤维细胞，但不由肝细胞产生，肝细胞是CRP的主要来源。本研究的目的是利用一种新的抗PTX3的单抗来研究PTX3在人类动脉粥样硬化病变中的表达模式。我们从急性心肌梗死患者和从尸检病例中采集的不同程度动脉粥样硬化的人主动脉组织中检测了含有动脉粥样硬化斑块成分的冠状动脉血栓。石蜡切片和冰冻切片免疫组织化学研究表明，在晚期动脉粥样硬化病变中，巨噬细胞(主要是泡沫细胞)表达PTX3。有趣的是，我们还清楚地观察到PTX3阳性的中性粒细胞渗入到动脉粥样硬化斑块中，这表明来源于中性粒细胞和巨噬细胞的PTX3在动脉粥样硬化形成中起着重要作用。版权所有(C)2008年大不列颠和爱尔兰病理学会。作者：John Wiley&Sons，Ltd.
Pentraxin 3 (PTX3) and C-reactive protein (CRP) are members of the pentraxin superfamily. PTX3 expression is induced in response to inflammatory signals, and is produced at sites of inflammation by several types of cell, primarily monocytes/macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells (SMCs), and fibroblasts, but is not produced by hepatocytes, which are a major source of CRP. The aim of our study was to investigate the expression pattern of PTX3 in human atherosclerotic lesions using a novel monoclonal antibody against PTX3. We examined coronary arterial thrombi containing an atherosclerotic plaque component removed from patients with acute myocardial infarction and human aortic tissues with various degrees of atherosclerosis sampled from autopsy cases. Immunohistochemical study of paraffin and frozen sections indicated that macrophages, mainly foam cells, expressed PTX3 in advanced atherosclerotic lesions. Interestingly, we also clearly observed PTX3-positive neutrophils infiltrating into atherosclerotic plaques, suggesting that PTX3 derived from neutrophils as well as macrophages plays an important role in atherogenesis. Copyright (C) 2008 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.