Reduction in heat-induced gastrointestinal hyperpermeability in rats by bovine colostrum and goat milk powders
Reduction in heat-induced gastrointestinal hyperpermeability in rats by bovine colostrum and goat milk powders
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DOI：
10.1152/japplphysiol.00295.2003
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发表时间：
2004-02-01
影响因子：
3.3
通讯作者：
Milne, C
中科院分区：
文献类型：
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作者：
Prosser, C;Stelwagen, K;Milne, C
将雄性Sprague-Dawley大鼠分配到三个饮食组[标准饮食（Cont; n = 8）、标准饮食加牛初乳粉（BColost 1.7 g/kg; n = 8）或山羊奶粉（GMilk 1.7 g/kg; n = 8）]中的一个，以确定这些补充剂降低由热诱导的胃肠道通透性过高的能力。将大鼠的核心体温升高至41.5 ℃，使对照组大鼠中Cr-51-EDTA从肠道向血液的转移增加了34倍（P < 0.05），表明胃肠道通透性增加。加热后，BColost组（Cont的27%）或GMilk组（Cont的10%）中转移到大鼠血液中的Cr-51-EDTA显著较少（P < 0.01），表明事先补充牛初乳或羊奶粉可显著降低热应激对胃肠道通透性的影响。BColost组的变化与GMilk组的变化无显著差异。牛初乳和山羊奶粉的保护作用的潜在机制可能涉及调节紧密连接的通透性，因为这两种粉末能够保持跨上皮电阻在Madin达比犬肾细胞与EGTA挑战相比，细胞保持在培养基中。结果表明，牛初乳粉可部分减轻高温对正常动物胃肠道通透性的影响。此外，羊奶粉与牛初乳粉同样有效，并且在胃肠道屏障功能受损的其他情况下两者都可能有益。
Male Sprague-Dawley rats were assigned to one of three dietary groups [ standard diet (Cont; n = 8), standard diet plus bovine colostrum powder (BColost 1.7 g/kg; n = 8), or goat milk powder (GMilk 1.7 g/kg; n = 8)] to determine the ability of these supplements to reduce gastrointestinal hyperpermeability induced by heat. Raising core body temperature of rats to 41.5degreesC increased transfer of Cr-51-EDTA from gut into blood 34-fold relative to the ambient temperature value ( P < 0.05) in the Cont group of rats, indicative of increased gastrointestinal permeability. Significantly less ( P < 0.01) Cr-51-EDTA was transferred into the blood of rats in either the BColost ( 27% of Cont) or GMilk group (10% of Cont) after heating, showing that prior supplementation with either bovine colostrum or goat milk powder significantly reduced the impact of heat stress on gastrointestinal permeability. The changes in the BColost group were not significantly different than those of the GMilk group. The potential mechanism of the protective effect of bovine colostrum and goat milk powders may involve modulation of tight junction permeability, because both powders were able to maintain transepithelial resistance in Madin Darby canine kidney cells challenged with EGTA compared with cells maintained in media only. The results show that bovine colostrum powder can partially alleviate the effects of hyperthermia on gastrointestinal permeability in the intact animal. Moreover, goat milk powder was equally as effective as bovine colostrum powder, and both may be of benefit in other situations where gastrointestinal barrier function is compromised.