Lectin receptors for endometrial H-type 1 antigen on goat conceptuses.
Lectin receptors for endometrial H-type 1 antigen on goat conceptuses.
复制标题
山羊受孕子宫内膜 H 型 1 抗原的凝集素受体。
DOI：
10.1111/j.1600-0897.2004.00190.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Newton,GR
中科院分区：
文献类型：
--
作者：
Woldesenbet,S;Garcia,R;Igbo,N;Leake,J;Lewis,SK;Newton,GR
问题：本研究的目的是确定山羊胚胎是否表达子宫内膜H-1型(HT1)抗原的凝集素样受体。研究方法：在胎盘的贴壁、粘连和附着阶段收集概念组织，并用免疫荧光显微镜进行评估。结果：三糖乳糖-N-岩藻五糖-1的概念染色在胎儿和母体组织的贴壁过程中强烈而均匀，但在妊娠第25天时发生变化，观察到大团块的强染色。Galectin-3的单抗在胚胎组织的贴合期不染色，但在第25天后观察到强烈的点状染色。仅在妊娠第17天检测到Lewis Y抗原的强均匀染色。结论：胚胎组织表达潜在的子宫内膜HT1抗原受体。在胎盘形成的早期阶段，碳水化合物-凝集素的相互作用可能促进子宫上皮细胞和滋养外胚层的顶面附着。
Problem:The goal of this study was to determine if caprine conceptuses express lectin‐like receptors for endometrial H‐type 1 (HT1) antigen.Method of study:Conceptus tissues were collected during the apposition, adhesion and attachment phases of placentation and evaluated using immunofluorescence microscopy.Results:Conceptus staining for the trisaccharide lacto‐N‐fucopentaose‐1 was strong and uniform during apposition of fetal and maternal tissues but changed by day 25 of pregnancy when large aggregates of intense staining were observed. Monoclonal antibodies to galectin‐3 did not stain conceptus tissue during the apposition phase but intense punctate staining was observed after day 25. Strong uniform staining for Lewis Y antigen was detected only on day 17 of pregnancy.Conclusion:Conceptus tissue expresses potential receptors for endometrial HT1 antigen. Carbohydrate–lectin interactions may facilitate attachment of the apical surfaces of uterine epithelial cells and trophectoderm during the early stages of placentation.