Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.
Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.
复制标题
DOI：
10.1099/0022-1317-49-6-525
复制
发表时间：
2000-06-01
影响因子：
3
通讯作者：
Fox, JG
中科院分区：
文献类型：
--
作者：
Chien, CC;Taylor, NS;Fox, JG
一种引起中国仓鼠卵巢（CHO）细胞和HeLa细胞进行性膨胀和死亡的细菌毒素，称为细胞致死性膨胀毒素（Cdt），已在几种致病细菌中鉴定出，包括弯曲杆菌属。（C. jejuni和C.大肠杆菌（Escherichia coli）、志贺氏菌（Shigella spp.）编码该毒素的基因被鉴定为三个相邻基因cdtA、cdtB和cdtC的簇。从五种肠肝螺杆菌（Helicobacter hepaticus，H. bilis，H.犬和两个新的螺杆菌属。分别从小鼠和土拨鼠中分离）通过变性PCR引物鉴定、克隆和测序。来自这些螺杆菌属的这些cdtB部分核苷酸序列与已知存在于其它细菌中的cdtB基因的相似性为：C.空肠肠杆菌58.3-64.8%;大肠杆菌52.3-57.8%，杜克雷嗜血杆菌53.4-58.4%，伴放线放线杆菌52.7-58.1%。从四个这些螺旋杆菌的细菌裂解物引起的HeLa细胞的特征性细胞致死性膨胀。流式细胞仪检测Cdt使细胞周期阻滞于G（2）/M期。结果表明这些螺杆菌属中存在毒素。属于CDT家族。
A bacterial toxin that causes progressive distension and death of Chinese hamster ovary (CHO) cells and HeLa cells termed cytolethal distending toxin (Cdt), has been identified in several diarrhoeagenic bacteria, including Campylobacter spp. (C. jejuni and C. coli), some pathogenic strains of Escherichia coli and Shigella spp. Genes encoding this toxin were identified as a cluster of three adjacent genes cdtA, cdtB and cdtC. Homologues of cdtB from five species of enterohepatic helicobacters (Helicobacter hepaticus, H. bilis, H. canis and two novel Helicobacter spp. isolated from mice and woodchuck, respectively) were identified by means of degenerative PCR primers, cloned and sequenced. The similarities of these partial cdtB nucleotide sequences from these Helicobacter spp, to those of cdtB genes known to be present in other bacteria were: C. jejuni, 58.3-64.8%; E. coli, 52.3-57.8%, Haemophilus ducreyi, 53.4-58.4% and Actinobacillus actinomycetemcomitans, 52.7-58.1%. Bacterial lysates from four of these helicobacters caused characteristic cytolethal distension of HeLa cells. Cdt caused cell cycle arrest at G(2)/M phase as measured by flow cytometry. The results demonstrated the presence of a toxin in these Helicobacter spp. belonging to the family of Cdt.