Augmentation by platelets of granulocyte aggregation in response to chemotaxins: studies utilizing an improved cell preparation technique
Augmentation by platelets of granulocyte aggregation in response to chemotaxins: studies utilizing an improved cell preparation technique
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血小板响应趋化素而增强粒细胞聚集：利用改进的细胞制备技术的研究
DOI：
10.1182/blood.v61.1.125.125
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发表时间：
1983
期刊：
影响因子：
20.3
通讯作者：
G. Schlag
中科院分区：
文献类型：
--
作者：
H. Redl;D. Hammerschmidt;G. Schlag
有大量证据表明，血小板和粒细胞(PMN)在休克肺损伤中起着重要作用。虽然认为这两种细胞的主要贡献是连续的，但体外观察表明，粒细胞和血小板之间的直接相互作用也可能放大组织损伤。因此，我们用等渗Percoll密度梯度分离PMN，研究了故意的血小板污染对PMN聚集的影响。1个血小板/PMN可增强PMN对N-甲酰甲基-亮氨酸-苯丙氨酸或激活补体的聚集，在16个血小板/PMN时达到最大(p<0.01)，形成大的混合聚集体。溶解的、阿司匹林的和消炎痛处理的血小板保留了它们的增强能力，通过凝胶过滤洗涤的血小板也是如此。在PMN制剂中添加组胺或5-羟色胺并不能模拟这种效果。这些血小板制剂都没有促进溶酶体酶的释放。我们得出结论，血小板通过形成巨大的混合PMN/血小板聚集体和产生一种不稳定的增强物质来增强PMN的聚集，这种物质的产生可能不依赖于血栓素的合成。我们认为，直接和顺序的血小板/中性粒细胞相互作用在休克组织损伤中可能是重要的。
Considerable evidence exists to suggest roles for both platelets and granulocytes (PMNs) in pulmonary injury in shock. While believing that the major contributions of the two cell types are sequential, in vitro observations suggested that direct interactions between granulocytes and platelets might also amplify tissue damage. Using isotonic Percoll density gradients to isolate PMNs, we therefore studied the effect of deliberate platelet contamination on PMN aggregation. PMN aggregation in response to N-formyl-met-leu-phe or activated complement was enhanced by the presence of 1 platelet/PMN, an effect that became maximal at 16 platelets/PMN (p less than 0.01); large mixed aggregates were formed. Lysed, aspirinated, and indomethacin-treated platelets retained their augmentative capacity, as did platelets washed by gel filtration. The effect was not mimicked by the addition of histamine or serotonin to PMN preparations. None of these platelet preparations augmented lysosomal enzyme release. We conclude that platelets augment PMN aggregation, both by forming giant mixed PMN/platelet aggregates and also by producing a labile augmentative substance, the production of which may be independent of thromboxane synthesis. We propose that direct as well as sequential platelet/PMN interactions may be important in tissue injury in shock.
体内补体诱导粒细胞聚集。
DOI：
--
发表时间：
1981
期刊：
The American journal of pathology
影响因子：
--
作者：
Hammerschmidt,DE;Harris,PD;Wayland,JH;Craddock,PR;Jacob,HS
通讯作者：
Jacob,HS