Identification of antimicrobial metabolites produced by a potential biocontrol Actinomycete strain A217
Identification of antimicrobial metabolites produced by a potential biocontrol Actinomycete strain A217
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DOI：
10.1111/jam.14548
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发表时间：
2020-01-24
影响因子：
4
通讯作者：
Han, L.
中科院分区：
文献类型：
--
作者：
He, H.;Hao, X.;Han, L.
目的提取并鉴定菌株A217的代谢产物及其抑菌谱和对多种植物病原菌的防治作用。方法与结果菌株A217为一株链霉菌属，与利埃霉素链霉菌最相似。抑菌谱试验表明，菌株A217对辣椒疫霉、灰霉病菌、菌核菌、尖孢镰刀菌、丁香假单胞菌和油菜黄单胞菌等几种植物病原真菌均有较强的抑菌作用。体内组织试验表明，菌株A217发酵液对菌核病菌的治疗和保护作用分别为49点、47点和61点，分别为60%。此外，A217发酵液对核桃黑斑病的防治效果在核桃叶和枝上分别达到79中心点33%和81中心点52%。在盆栽试验中，发酵液对辣椒疫霉病具有较强的防治作用（68中心点29%），对辣椒疫霉病有较好的抑菌和防病效果。采用柱层析和高效液相色谱法从菌株A217发酵液中分离得到具有抗真菌和抗菌活性的化合物。利用质谱和核磁共振进行化学和结构分析，证实这些化合物为1h -吡咯-2-羧酸和1h -吡咯-2-羧酰胺。化合物1h -吡咯-2-羧酸对菌核病菌和辣椒病菌的EC50值分别为20中心点13和50中心点36 μ g ml（-1）。化合物1h -吡咯-2-carboxamide2对不同植物病原菌均有明显的抑菌活性。测定了丁香、野田葵和野田葵的MIC值。Jugiandis分别为7个中心点5、30和15个中心点0 μ g ml（-1）。结论放线菌A217发酵产物抑菌谱广，对多种植物病原真菌和细菌具有良好的抑菌活性。研究的意义和影响本研究揭示了一种新的产抗菌链霉菌菌株及其作为植物病害生物防治剂的潜在应用前景。
Aims To extract and identify the metabolites of strain A217 as well as its antifungal spectrum and control effect on various plant pathogens. Methods and Results Strain A217 was identified as a Streptomyces sp. which was most similar to Streptomyces lienomycini. An antimicrobial spectrum test indicated that strain A217 inhibited several plant pathogenic fungi and strong antibacterial effect such as Phytophthora capsici, Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium oxysporum, Pseudomonas syringae and Xanthomonas campestris. An in vivo tissue test demonstrated that the fermentation broth of strain A217 exerted therapeutic and protective effects of 49 center dot 47 and 61 center dot 60% respectively, on S. sclerotiorum. Additionally, the fermentation broth of A217 exerted control effects on walnut black spot disease in walnut leaves and branches amounting to 79 center dot 33 and 81 center dot 52% respectively. In a pot experiment, the fermentation broth exhibited a stronger protective and control effect (68 center dot 29%), as well as better bacteriostatic and disease control effects on Phytophthora blight of pepper, compared with Metalaxyl. Compounds possessing antifungal and antibacterial activities were obtained from the fermentation broth of strain A217, using column chromatography and HPLC. Chemical and structural analyses conducted using MS and nuclear magnetic resonance confirmed that these compounds were 1H-pyrrole-2-carboxylic acid and 1H-pyrrole-2-carboxamide. The EC50 values of compound 1H-pyrrole-2-carboxylic acid1 for S. sclerotiorum and P. capsici were 20 center dot 13 and 50 center dot 36 mu g ml(-1) respectively. Compound 1H-pyrrole-2-carboxamide2 showed significant antibacterial activity against different plant pathogenic bacteria. The MIC values of P. syringae, X. campestris and X. campestris pv. jugiandis were 7 center dot 5, 30 and 15 center dot 0 mu g ml(-1) respectively. Conclusions Actinomyces A217 fermentation products have a broad spectrum of bacteriostasis, and have good bacteriostasis activity to many plant pathogenic fungi and bacteria. Significance and Impact of the Study The present study revealed a new antimicrobial producing strain of Streptomyces and its potential application as a biological control agent for plant diseases.