Dermacentor andersoni:: effects of repeated infestations on lymphocyte proliferation, cytokine production, and adhesion-molecule expression by BALB/c mice
Dermacentor andersoni:: effects of repeated infestations on lymphocyte proliferation, cytokine production, and adhesion-molecule expression by BALB/c mice
复制标题
DOI：
10.1080/00034980120059081
复制
发表时间：
2001-06-01
影响因子：
--
通讯作者：
Wikel, SK
中科院分区：
文献类型：
--
作者：
Macaluso, KR;Wikel, SK
本文研究了安氏革蜱（Dermacentorandersoni）反复感染对BALB/c小鼠淋巴细胞功能的影响。在体外增殖反应伴刀豆球蛋白-A或唾液腺分子，细胞因子的生产，和两个粘附分子白细胞功能相关抗原-1（LFA-1）和非常晚激活-4（VLA-4）的表达进行了研究。此外，D.首次和第二次感染后，小鼠T淋巴细胞对刀豆球蛋白A的增殖反应明显受到抑制，而在蜱唾液腺抗原存在下，小鼠T淋巴细胞的增殖反应明显增强。两次感染后，白细胞介素-2的产生显着下降，但干扰素-γ保持不变。白细胞介素-4和白细胞介素-10的生产显着增强后，第一次和第二次感染的感染小鼠。感染小鼠淋巴细胞LFA-1和VLA-4的表达受到抑制。此外，唾液腺提取物和D。Andersoni减少了来自蜱未感染小鼠的淋巴细胞对这两种粘附分子的体外表达。
The effects of repeated infestations with Dermacentor andersoni nymphs on the lymphocyte functions of BALB/c mice were investigated. The in-vitro proliferation responses to concanavalin-A or salivary-gland molecules, the production of cytokines, and the expression of two adhesion molecules-leucocyte function-associated antigen-1 (LFA-1) and very late activation-4 (VLA-4) -were all studied. In addition, the ability of salivary-gland extract or saliva from D. andersoni to modulate expression of lymphocyte adhesion molecules in vitro was determined.The proliferative responses of T-lymphocytes to concanavalin-A were significantly suppressed after first and second infestations, and significant increases in lymphocyte proliferation in the presence of tick salivary-gland antigen were observed in infested mice. After two infestations, production of interleukin-2 was significantly decreased but that of interferon-gamma remained unchanged. Production of interleukin-4 and interleukin-10 was significantly enhanced in infested mice after both the first and second infestations. Expression of LFA-1 and VLA-4 by lymphocytes from infested mice was suppressed. Furthermore, both a salivary-gland extract and the saliva of D. andersoni reduced the in-vitro expression of both of these adhesion molecules by lymphocytes from tick-naive mice.