Lectin‐induced rearrangement of an immature hematopoietic cell surface marker
Lectin‐induced rearrangement of an immature hematopoietic cell surface marker
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凝集素诱导的未成熟造血细胞表面标记物的重排
DOI：
10.1002/jcp.1041100304
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发表时间：
1982
影响因子：
5.6
通讯作者：
R. Schlegel
中科院分区：
文献类型：
--
作者：
B. M. Phelps;Patrick Williamson;R. Schlegel
进行实验以检查无序膜结构域在造血细胞表面分化中可能的生理作用。造血干细胞系416 B已显示结合染料mercury 540（MC 540），所述染料mercury 540是可对脂质双层的无序区域特异的荧光探针（威廉姆森（Williamson）等人，1981年）。凝集素伴刀豆球蛋白A（Con A）和麦胚凝集素（WGA）的表面受体在416 B细胞表面呈斑片状分布，这与MC 540结合区的分布相对应。这些细胞与两种凝集素中的任一种适当孵育导致诱导形成帽。MC540的结合区域和其他凝集素的受体通过该过程定位于膜的相同区域。与无序脂质结构域相关的表面糖蛋白的这种协调重排可能在不同表面分子的共封端（Raz和Bucana，1980）或分化相关的细胞表面重排中起作用（Schlegel等人，1980年）。
Experiments were carried out to examine the possible physiological role of disordered membrane domains in hematopoietic cell surface differentiation. The hematopoietic stem cell line 416B has been shown to bind the dye merocyanine 540 (MC540), a fluorescent probe which may be specific for disordered regions of lipid bilayers (Williamson et al., 1981). The surface receptors for the lectins Concanavalin A (Con A) and wheat germ agglutinin (WGA) exhibit patchy distributions on the surface of 416B cells which correspond to the distribution of MC540 binding regions. Appropriate incubation of these cells with either of the two lectins results in the induced formation of a cap. The binding regions for MC540 and the receptors for the other lectin become localized to the same region of the membrane by this process. Such coordinated rearrangements of surface glycoproteins associated with disordered lipid domains may play a role in the cocapping of disparate surface molecules (Raz and Bucana, 1980) or in differentiation‐related cell surface rearrangements (Schlegel et al., 1980).