Identification of a keratinocarcinoma cell line expressing AQP3
Identification of a keratinocarcinoma cell line expressing AQP3
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DOI：
10.1042/bc20040127
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发表时间：
2006-02-01
影响因子：
2.7
通讯作者：
Ishibashi, K
中科院分区：
文献类型：
--
作者：
Nakakoshi, M;Morishita, Y;Ishibashi, K
背景资料。对AQP3缺失小鼠的研究表明，皮肤中的AQP3（水通道蛋白3）对皮肤水分至关重要，导致皮肤干燥加速。预防皮肤干燥不仅从美容角度而且从临床角度来看都很重要。原代角质形成细胞培养对于筛选调节AQP3表达的物质是很麻烦的。发现人角化癌细胞株表达AQP3 mRNA和蛋白，山梨醇对高渗刺激有应答，提示该细胞株AQP3的表达正常调控。该细胞系也表达1型角化细胞转谷氨酰胺酶基因。10(-6) m的全反式维甲酸对AQP3的表达没有影响，同样，维甲酸在1%浓度下也没有增加大鼠皮肤AQP3的表达。该细胞系可用于筛选调节AQP3表达的候选物质。
Background information. AQP3 (aquaporin 3) in the skin is important for skin moisture as demonstrated by the studies of AQP3-null mice, which have accelerated skin drying. Prevention of dry skin is important not only from a cosmetic but also from a clinical point of view. Primary keratinocyte cultures are cumbersome for screening substances that modulate AQP3 expression.Results. A human keratocarcinoma cell line was found to express AQP3 mRNA and protein, which responded to hypertonic stimulation with sorbitol, suggesting that the AQP3 expression is normally regulated in this cell line. This cell line also expressed the type 1 keratinocyte transglutaminase gene. The AQP3 expression was unaffected by all-trans-retinoic acid Up to 10(-6) M. Similarly, the retinoic acid did not increase the AQP3 expression up to 1% concentration in rat skin.Conclusion. This cell line is useful for the screening of candidate substances that modulate AQP3 expression.