Survival prediction of gastric cancer by a seven-microRNA signature
Survival prediction of gastric cancer by a seven-microRNA signature
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DOI：
10.1136/gut.2008.175497
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发表时间：
2010-05-01
期刊：
影响因子：
24.5
通讯作者：
Fan, Daiming
中科院分区：
文献类型：
--
作者：
Li, Xiaohua;Zhang, Ying;Fan, Daiming
目的几个微阵列研究已经报道了将癌症患者分类为不同预后组的microRNA(MiRNA)表达特征。目前还没有研究评估miRNA表达模式与胃癌预后之间的关系。在这项研究中，我们开发了一个与胃癌患者生存密切相关的七个miRNA信号。患者和方法通过实时荧光RT-PCR分析100名胃癌患者的miRNA表达谱，这些患者被随机分配到训练组和测试组。用COX比例风险回归和风险-分数分析确定训练集中一组独立于阶段的7-miRNA信号，可以对预后有显著差异的患者进行分类。结果我们鉴定了7个miRNA标记(miR-10b、miR-21、miR-223、miR-338、let-7a、miR-30a-5p、miR-126)对胃癌患者的总生存期(p=0.0009)和无复发生存期(p=0.0005)进行了预测。多因素分析显示，风险特征是总生存率(HR=3.046；95%CI，1.246~7.445，p=0.015)和无复发生存率(HR=3.337；95%CI，1.298~8.580，p=0.012)的独立预测因子。此外，在50名患者和60名独立患者的测试集中验证了这一7-miRNA特征的预测价值。结论我们的7-miRNA特征与胃癌患者的无复发和总生存率密切相关。预后标志可能适用于未来有关治疗的决定。
Aims Several microarray studies have reported microRNA (miRNA) expression signatures that classify cancer patients into different prognostic groups. No study has evaluated the association between miRNA expression patterns and gastric cancer prognosis. In this study, we developed a seven-miRNA signature that is closely associated with survival of patients with gastric cancer.Patients and methods MiRNA expression profile was analysed by real-time RT-PCR in 100 gastric cancer patients, which were randomly assigned to either the training set or the testing set. Cox proportional hazard regression and risk-score analysis were used to identify a stage-independent set of seven-miRNA signature in the training set that could classify patients with significantly different prognosis. This miRNA signature was further validated by the testing set and an independent cohort 60 patients.Results We have identified a seven-miRNA signature (miR-10b, miR-21, miR-223, miR-338, let-7a, miR-30a-5p, miR-126) for overall survival (p = 0.0009) and relapse-free survival (p = 0.0005) of gastric cancer patients. Multivariate analysis shown that the risk signature was an independent predictor of overall survival (HR = 3.046; 95% CI, 1.246 to 7.445, p = 0.015) and relapse-free survival (HR = 3.337; 95% CI, 1.298 to 8.580, p = 0.012). Furthermore, the predictive value of this seven-miRNA signature was validated in the testing set of 50 patients and an independent set of 60 patients.Conclusion Our seven-miRNA signature is closely associated with relapse-free and overall survival among patients with gastric cancer. The prognostic signature could be applicable to future decisions concerning treatment.