FERTILIZATION AND ZYGOTE DEVELOPMENT IN THE BINUCLEATE DINOFLAGELLATE PERIDINIUM-BALTICUM (PYRRHOPHYTA)
FERTILIZATION AND ZYGOTE DEVELOPMENT IN THE BINUCLEATE DINOFLAGELLATE PERIDINIUM-BALTICUM (PYRRHOPHYTA)
复制标题
DOI：
10.2307/2444528
复制
发表时间：
1989-07-01
影响因子：
3
通讯作者：
COX, ER
中科院分区：
文献类型：
--
作者：
CHESNICK, JM;COX, ER
波罗的海多甲藻（Peridinium balticum）（甲藻门）作为非光合甲藻宿主和含叶绿素c的藻类之间的共生体而存在。据推测，波罗的海原甲藻是原始非光合甲藻之间的进化联系。本研究首次记录了这种不寻常的甲藻有性生殖的受精前和受精后事件。光学显微镜和透射电镜观察表明，配子类似于营养细胞，但其叶绿体的组织。配子的融合发生在特定的方向，即，顶端到沟区。在胞浆融合之前融合对之间存在完整的膜，提示受精过程中板的酶消化。这层膜的破裂触发宿主的胞浆融合和核融合，随后是藻类共生体的胞浆融合和核融合。本文讨论了配子形成、受精和合子发育的细胞过程。本次调查的结果表明，一个同步的有性生殖周期的共生体和它的dingoflagellate主机已经演变。
Peridinium balticum (Pyrrhophyta) exists as a symbiosis between a nonphotosynthetic dinoflagellate host and a chlorophyll c-containing alga. It is hypothesized that P. balticum is an evolutionary link between primitive nonphotosynthetic dinoflagellates. This study documents pre- and postfertilization events of sexual reproduction in this unusual dinoflagellate for the first time. Light microscopy and TEM observation showed that gametes resemble vegetative cells except in the organization of their chloroplasts. Fusion of gametes occurred in a specific orientation, i.e., apical to sulcal area. The presence of an intact membrane between fusing pairs prior to plasmogamy was suggestive of enzymatic digestion of plates during fertilization. Rupture of this membrane triggers plasmogamy and karyogamy of the host, followed by that of the algal symbiont. A discussion of the cellular process involved in gamete formation, fertilization, and zygote development is presented. The results of this investigation demonstrate that a synchronous sexual reproduction cycle has evolved for the symbiont and its dingoflagellate host.