Prediction and optimization of the separation of metal cations by capillary electrophoresis with indirect UV detection.
Prediction and optimization of the separation of metal cations by capillary electrophoresis with indirect UV detection.
复制标题
通过毛细管电泳和间接紫外检测来预测和优化金属阳离子的分离。
DOI：
10.1016/0021-9673(94)85087-9
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Khaledi,MG
中科院分区：
文献类型：
--
作者：
Quang,C;Khaledi,MG
研究了金属离子在毛细管电泳-间接紫外检测中的迁移行为。一个数学模型被用来与两个背景电解质的因素：pH值和浓度的络合剂的金属阳离子的电泳迁移率。咪唑，其具有接近金属阳离子的电泳迁移率，被用作显色剂，和2-羟基异丁酸（HIBA）被用作络合剂。通过计算机模拟，14种金属阳离子的电泳迁移率预测的基础上，在pH值和HIBA浓度的因素空间内的五个初始实验。金属阳离子的电泳迁移率预测值和实测值之间观察到良好的一致性。随后，成功地优化了金属阳离子的复杂混合物的分离。14种金属阳离子，包括碱金属，碱土金属和过渡金属（II）阳离子的完全分离在4分钟内实现。此外，其他可视化剂和络合剂的有效性进行了检查，用于金属阳离子的分离。
The migration of metal cations in capillary electrophoresis with indirect UV detection was investigated. A mathematical model was used to relate the electrophoretic mobility of a metal cation with two background electrolyte factors: the pH and the concentration of a complexing agent. Imidazole, which has an electrophoretic mobility close to those of metal cations, was used as the visualization agent, and 2-hydroxyisobutyric acid (HIBA) was used as the complexing agent. Through computer modeling, the electrophoretic mobilities of fourteen metal cations were predicted on the basis of five initial experiments within the factor space of pH and HIBA concentration. Good agreement was observed between the predicted electrophoretic mobilities and the observed values of the metal cations. Subsequently, the separation of a complex mixture of metal cations was successfully optimized. A complete separation of 14 metal cations including alkali, alkaline earth and transition metal(II) cations was achieved within 4 min. In addition, the effectiveness of other visualization agents and complexing agents for the separation of metal cations was examined.