Defective vasculogenesis in systemic sclerosis
Defective vasculogenesis in systemic sclerosis
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DOI：
10.1016/s0140-6736(04)16853-0
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发表时间：
2004-08-14
期刊：
影响因子：
168.9
通讯作者：
Ikeda, Y
中科院分区：
文献类型：
--
作者：
Kuwana, M;Okazaki, Y;Ikeda, Y
系统性硬化症的典型血管特征包括毛细血管密度降低和血管闭塞。成人血管的形成和修复涉及血管发生，其通过募集骨髓来源的循环内皮前体（CEP）介导。我们调查是否血管发生受损的患者systemicsclerosis.Methods外周血中获得11例系统性硬化症，11例类风湿性关节炎，和11名健康对照。潜在影响CEP数的因素在三组之间匹配。通过细胞分选和三色流式细胞术定量CEP（鉴定为CD 34、CD 133和血管内皮生长因子2型受体阳性的循环细胞）。通过ELISA测量血管生成因子的循环浓度。CEP分化为内皮细胞的潜力通过体外成熟治疗后血管性血友病因子的上调来评估。结果系统性硬化症患者的CEP绝对数量远低于类风湿性关节炎患者或健康对照组（中位数274 [IQR 178-395] vs 1154 [653-1524]和1074 [713-1186]）。每20 mL外周血中，分别为：
Background Typical vascular features of systemic sclerosis include low capillary density and vascular obliteration. The formation and repair of blood vessels in adults involve vasculogenesis, which is mediated through the recruitment of bone-marrow-derived circulating endothelial precursors (CEP). We investigated whether vasculogenesis is impaired in patients with systemic sclerosis.Methods Peripheral blood was obtained from 11 patients with systemic sclerosis, 11 with rheumatoid arthritis, and 11 healthy controls. Factors potentially affecting the CEP number were matched among the three groups. CEP (identified as circulating cells positive for CD34, CD133, and the type 2 receptor for vascular endothelial growth factor) were quantified by cell sorting and three-colour flow cytometry. The circulating concentrations of angiogenic factors were measured by ELISA. The potential of CEP to differentiate into endothelial cells was assessed by the upregulation of von Willebrand factor after in-vitro maturation treatment.Findings The absolute number of CEP was much lower in patients with systemic sclerosis than in patients with rheumatoid arthritis or healthy controls (median 274 [IQR 178-395] vs 1154 [653-1524] and 1074 [713-1186] per 20 mL peripheral blood, respectively; p