Self-organization and advective transport in the cell polarity formation for asymmetric cell division
Self-organization and advective transport in the cell polarity formation for asymmetric cell division
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DOI：
10.1016/j.jtbi.2015.06.032
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发表时间：
2015-10-07
影响因子：
2
通讯作者：
Shibata, Tatsuo
中科院分区：
文献类型：
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作者：
Lee, Sungrim Seirin;Shibata, Tatsuo
细胞膜蛋白的前后（AP）极性形成在决定细胞不对称性方面起着至关重要的作用，这不仅取决于多种遗传过程，还取决于生化和生物物理相互作用。秀丽隐杆线虫胚胎的 AP 形成机制分为三个过程：（i）后部和前部蛋白质的膜结合和解离，（ii）扩散到膜和细胞质中，以及（iii）肌动球蛋白皮质收缩诱导的活跃皮质和细胞质流动。我们利用后部蛋白质的自招募模型探索了对称性破坏和 AP 极性形成的机制。我们发现，AP 极性模式在极性蛋白的总质量和细胞质到膜的扩散比的大范围内建立。我们还表明，建立阶段膜和细胞质中的平流运输会影响后域建立和定位的最佳时间间隔，并通过减少后域数量对初始条件的敏感性来提高 AP 极性形成的鲁棒性。我们还证明，总质量与细胞大小的适当比率可以稳健地调节后域的长度尺度。 (C) 2015 Elsevier Ltd. 保留所有权利。
Anterior-Posterior (AP) polarity formation of cell membrane proteins plays a crucial role in determining cell asymmetry, which depends not only on the several genetic process but also biochemical and biophysical interactions. The mechanism of AP formation of Caenorhabditis elegans embryo is characterized into the three processes: (i) membrane association and dissociation of posterior and anterior proteins, (ii) diffusion into the membrane and cytosol, and (iii) active cortical and cytoplasmic flows induced by the contraction of the acto-myosin cortex. We explored the mechanism of symmetry breaking and AP polarity formation using self-recruitment model of posterior proteins. We found that the AP polarity pattern is established over wide range in the total mass of polarity proteins and the diffusion ratio in the cytosol to the membrane. We also showed that the advective transport in both membrane and cytosol during the establishment phase affects optimal time interval of establishment and positioning of the posterior domain, and plays a role to increase the robustness in the AP polarity formation by reducing the number of posterior domains for the sensitivity of initial conditions. We also demonstrated that a proper ratio of the total mass to cell size robustly regulate the length scale of the posterior domain. (C) 2015 Elsevier Ltd. All rights reserved.