Normal testis determination in the mouse depends on genetic interaction of a locus on chromosome 17 and the Y chromosome.
Normal testis determination in the mouse depends on genetic interaction of a locus on chromosome 17 and the Y chromosome.
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小鼠正常睾丸的测定取决于 17 号染色体和 Y 染色体上基因座的遗传相互作用。
DOI：
10.1093/genetics/123.1.173
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发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Eicher,EM
中科院分区：
文献类型：
--
作者：
Washburn,LL;Eicher,EM
我们以前描述了一个位点上的染色体（Chr）17的小鼠，这是至关重要的正常睾丸发育。该基因座被命名为"T相关性反转"（Tas），因为它与显性短尾畸形等位基因发夹尾（Thp）分离，并导致C57 BL/6J XY，Thp/+个体的性腺发育为卵巢或卵巢睾丸而不是睾丸。为了阐明Tas的遗传，我们研究了另一种短尾突变T-Orleans（TOr1）对性腺发育的影响。我们发现，如果Y染色体来源于AKR/J近交系，则C57 BL/6J XY、Thp/+和TOr 1/+小鼠的性腺发育卵巢组织，而在存在来源于C57 BL/6J近交系的Y染色体的情况下发生正常的睾丸发育。从这些观察中，我们得出结论：（1）Tas位于Chr 17上与Thp和TOrl相关缺失共同的区域，以及（2）AKR/J近交系携带的Y连锁睾丸决定基因Tdy与C57 BL/6 J近交系不同。我们认为，在哺乳动物中，Tdy是不是唯一的睾丸决定因素，因为常染色体位点必须与Tdy正常睾丸发育的遗传兼容。
We previously described a locus on chromosome (Chr) 17 of the mouse that is critical for normal testis development. This locus was designated "T-associated sex reversal" (Tas) because it segregated with the dominant brachyury allele hairpin tail (Thp) and caused gonads of C57BL/6J XY, Thp/+ individuals to develop as ovaries or ovotestes rather than as testes. To clarify the inheritance of Tas, we investigated the effects of T-Orleans (TOrl), another brachyury mutation, on gonad development. We found that gonads of C57BL/6J XY, Thp/+ and TOrl/+ mice develop ovarian tissue if the Y chromosome is derived from the AKR/J inbred strain, whereas normal testicular development occurs in the presence of a Y chromosome derived from the C57BL/6J inbred strain. From these observations we conclude that: (1) Tas is located in a region on Chr 17 common to the deletions associated with Thp, and TOrl, and (2) the Y-linked testis determining gene, Tdy, carried by the AKR/J inbred strain differs from that of the C57BL/6J inbred strain. We suggest that in mammals Tdy is not the sole testis determinant because autosomal loci must be genetically compatible with Tdy for normal testicular development.