Improved procedure for protein binder analysis in mural painting by LC-ESI/Q-q-TOF mass spectrometry: detection of different milk species by casein proteotypic peptides
Improved procedure for protein binder analysis in mural painting by LC-ESI/Q-q-TOF mass spectrometry: detection of different milk species by casein proteotypic peptides
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DOI：
10.1007/s00216-009-3183-0
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发表时间：
2009-12-01
影响因子：
4.3
通讯作者：
Arcari, Paolo
中科院分区：
文献类型：
--
作者：
Chambery, Angela;Di Maro, Antimo;Arcari, Paolo
应用于文化遗产领域的诊断技术是科学调查的一个非常重要的方面。最近，蛋白质组学方法的基础上，质谱结合传统的光谱方法已被用于绘画分析，产生有前途的结果粘合剂的蛋白质鉴定。在目前的工作中，一个改进的程序的基础上LC-ESI/Q-q-TOF串联质谱鉴定的蛋白质结合剂已开发的分子表征的样品从早期的二十-世纪壁画从圣Dimitar大教堂在维丁，保加利亚。根据蛋彩画的传统老配方，蛋白质组学研究鉴定出了鸡蛋白色和蛋黄蛋白。此外，除了鸡蛋成分外，还发现了酪蛋白的存在，从而表明使用牛奶作为结合介质、固定剂或稳定剂。此外，第一次，有能力区分牛奶来源的基础上的α酪蛋白型肽的报告，这是诊断一个给定的物种，从而打开有趣的视角，在艺术和考古领域。
Diagnostic techniques applied to the field of cultural heritage represent a very important aspect of scientific investigation. Recently, proteomic approaches based on mass spectrometry coupled with traditional spectroscopic methods have been used for painting analysis, generating promising results for binder's protein identification. In the present work, an improved procedure based on LC-ESI/Q-q-TOF tandem mass spectrometry for the identification of protein binders has been developed for the molecular characterization of samples from an early-twentieth-century mural painting from the St. Dimitar Cathedral in Vidin, Bulgaria. The proteomic investigation has led to the identification of both egg white and egg yolk proteins, according to traditional old recipes for tempera paintings. In addition, beyond the egg components, the presence of caseins was also revealed, thus suggesting the use of milk as binding medium, fixative or stabilising agent. Furthermore, for the first time, the capability to discriminate the milk origin on the basis of alpha casein proteotypic peptides is reported, that are diagnostic for a given species, thus opening interesting perspectives in art and archaeological fields.