Substance P in human cerebrospinal fluid
Substance P in human cerebrospinal fluid
复制标题
人脑脊液中的 P 物质
DOI：
10.1212/wnl.30.12.1280
复制
发表时间：
1980
期刊：
影响因子：
9.9
通讯作者：
T. Chase
中科院分区：
文献类型：
--
作者：
J. Nutt;E. Mroz;S. Leeman;Adrian C Williams;W. Engel;T. Chase
用放射免疫分析法测定了人腰段脑脊液（CSF）中P物质（SP）含量。18名神经系统正常成人的CSF中存在P物质样免疫反应性（SPLI），浓度范围为2.9至11.1 fmol/ml，平均值为7.0 ± 0.6 fmol/ml（平均值± SE）。略多于一半的CSF-SPLI与合成SP在Sephadex G-25上共层析。在腰椎穿刺取出的前30 ml CSF中，CSF-SPLI浓度无明显梯度。在患有亨廷顿病、帕金森综合征、各种运动障碍、进行性核上性麻痹、肌病和肌萎缩侧索硬化症的患者中，CSF-SPLI的平均浓度与正常人无显著差异。神经病变或多系统萎缩（Shy-Drager综合征）患者的平均CSF-SPLI浓度显著降低。这些观察结果表明，腰椎CSF-SPLI主要来自脊髓，神经根，或背根神经节，影响这些结构的病理过程可能会反映CSF-SPLI水平降低。
Substance P (SP), a putative peptide neurotransmitter, was measured in human lumbar cerebrospinal fluid (CSF) by radioimmunoassay. Substance P-like immunoreactivity (SPLI) was present in the CSF of 18 neurologically normal adults in concentrations ranging from 2.9 to 11.1 fmol per milliliter, with a mean of 7.0 ± 0.6 fmol per milliliter (mean ± SE). Slightly more than half of the CSF-SPLI cochromatographed with synthetic SP on Sephadex G-25. There was no apparent gradient in CSF-SPLI concentration over the first 30 ml of CSF removed by lumbar puncture. Mean concentrations of CSF-SPLI in patients with Huntington disease, parkinsonism, miscellaneous dyskinesias, progressive supranuclear palsy, myopathy, and amyotrophic lateral sclerosis did not differ significantly from normal. Patients with neuropathy or multiple-system atrophy (Shy-Drager syndrome) had significantly reduced mean CSF-SPLI concentrations. These observations suggest that lumbar CSF-SPLI arises largely from spinal cord, nerve roots, or dorsal root ganglia, and that pathologic processes affecting these structures may be reflected by reduced levels of CSF-SPLI.