IMMUNOHISTOCHEMICAL STUDIES ON COMPLEX-I, COMPLEX-II, COMPLEX-III, AND COMPLEX-IV OF MITOCHONDRIA IN PARKINSONS-DISEASE
IMMUNOHISTOCHEMICAL STUDIES ON COMPLEX-I, COMPLEX-II, COMPLEX-III, AND COMPLEX-IV OF MITOCHONDRIA IN PARKINSONS-DISEASE
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DOI：
10.1002/ana.410300409
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发表时间：
1991-10-01
影响因子：
11.2
通讯作者：
MIZUNO, Y
中科院分区：
文献类型：
--
作者：
HATTORI, N;TANAKA, M;MIZUNO, Y
我们使用针对线粒体电子传递系统复合物I、II、III和IV的抗血清免疫组化检测了8例帕金森病患者的黑质。在帕金森氏病患者中，相当比例的黑神经细胞对复合物I抗体的染色减少。染色减少的神经元占黑化神经元的12.7% ~ 74.1%。虽然在对照组中也观察到复合物I免疫染色降低的神经元，但其在神经神经元中的比例明显小于帕金森病患者。复合体III和IV染色正常。3例帕金森病患者黑质复合体II染色降低。这些结果与我们的发现一致，即帕金森病患者纹状体中线粒体DNA中四个亚基的基因编码存在缺失。
We examined the substantia nigra of 8 patients with Parkinson's disease immunohistochemically using antisera against complexes I, II, III, and IV of the mitochondrial electron transport system. In the patients with Parkinson's disease, a fair proportion of the nigral neurons showed reduced staining against the complex I antibody. The proportion of the neurons with reduced staining ranged from 12.7 to 74.1% of the melanized nigral neurons. Although neurons with reduced immunostaining for complex I were also observed in control subjects, the proportion among the nigral neurons was significantly smaller than in parkinsonian patients. Staining for complexes III and IV appeared normal. Staining of substantia nigra for complex II was decreased in 3 parkinsonian patients. These results are consistent with our findings that there is a deletion of gene coding for the four subunits in the mitochondrial DNA located in the striata of parkinsonian patients.