Analysis of EF-hand-containing proteins in Arabidopsis.
Analysis of EF-hand-containing proteins in Arabidopsis.
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DOI：
10.1186/gb-2002-3-10-research0056
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发表时间：
2002-09-23
期刊：
影响因子：
12.3
通讯作者：
Reddy AS
中科院分区：
文献类型：
--
作者：
Day IS;Reddy VS;Shad Ali G;Reddy AS
在植物的4类钙结合蛋白中，有3类含有钙结合EF-手结构域。最多鉴定出250种可能具有EF手的蛋白质。多种蛋白质，包括酶、参与转录和翻译的蛋白质、蛋白质和核酸结合蛋白以及大量未知蛋白质，都具有一个或多个推定的EF手。在植物中，钙（Ca 2+）已成为一种重要的信使，介导许多激素和环境信号的作用，包括生物和非生物胁迫。许多不同的信号升高细胞溶质钙浓度（[Ca 2 +]cyt），这反过来被认为是通过Ca 2+结合蛋白调节细胞和发育过程。在植物的四类钙结合蛋白中，有三类含有钙结合EF-手基序。该基序是一种保守的螺旋-环-螺旋结构，可以结合单个Ca 2+离子。为了鉴定拟南芥中所有的EF-手蛋白，我们分析了其完整的基因组序列编码EF-手蛋白。最多鉴定出250种可能具有EF手的蛋白质。多种蛋白质，包括酶、参与转录和翻译的蛋白质、蛋白质和核酸结合蛋白以及大量未知蛋白质，都具有一个或多个推定的EF手。系统发育分析确定了包含一些蛋白质家族的六个主要群体。EF-手基序在多种蛋白质中的存在与Ca 2+参与调节许多细胞和发育过程是一致的。到目前为止，只有47个可能的250 EF手蛋白已在文献中报道。我们在许多未表征的EF-手蛋白中鉴定的各种结构域应该有助于阐明它们的细胞作用。我们的分析表明，Ca 2+信使系统在植物中被广泛使用，EF-手蛋白可能是介导Ca 2+作用的关键转换器。
Three out of the four classes of Ca2+-binding proteins in plants contain Ca 2+ -binding EF-hand motif(s). A maximum of 250 proteins possibly having EF-hands were identified. Diverse proteins, including enzymes, proteins involved in transcription and translation, protein- and nucleic-acid-binding proteins and a large number of unknown proteins, have one or more putative EF-hands. In plants, calcium (Ca2+) has emerged as an important messenger mediating the action of many hormonal and environmental signals, including biotic and abiotic stresses. Many different signals raise cytosolic calcium concentration ([Ca2+]cyt), which in turn is thought to regulate cellular and developmental processes via Ca2+-binding proteins. Three out of the four classes of Ca2+-binding proteins in plants contain Ca2+-binding EF-hand motif(s). This motif is a conserved helix-loop-helix structure that can bind a single Ca2+ ion. To identify all EF-hand-containing proteins in Arabidopsis, we analyzed its completed genome sequence for genes encoding EF-hand-containing proteins. A maximum of 250 proteins possibly having EF-hands were identified. Diverse proteins, including enzymes, proteins involved in transcription and translation, protein- and nucleic-acid-binding proteins and a large number of unknown proteins, have one or more putative EF-hands. Phylogenetic analysis identified six major groups that contain some families of proteins. The presence of EF-hand motif(s) in a diversity of proteins is consistent with the involvement of Ca2+ in regulating many cellular and developmental processes. Thus far, only 47 of the possible 250 EF-hand proteins have been reported in the literature. Various domains that we identified in many of the uncharacterized EF-hand-containing proteins should help in elucidating their cellular role(s). Our analyses suggest that the Ca2+ messenger system is widely used in plants and that EF-hand-containing proteins are likely to be the key transducers mediating Ca2+ action.
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影响因子：
11.4
作者：
Czempinski, K;Zimmermann, S;MullerRober, B
通讯作者：
MullerRober, B
影响因子：
4.9
作者：
Furuichi, T;Cunningham, KW;Muto, S
通讯作者：
Muto, S
DOI：
10.1073/pnas.97.11.6224
发表时间：
2000-05-23
影响因子：
11.1
作者：
Hwang, I;Sze, H;Harper, JF
通讯作者：
Harper, JF
影响因子：
5.1
作者：
Hong, Y;Takano, M;Chua, NH
通讯作者：
Chua, NH
影响因子：
7.4
作者：
BARTLING, D;BULTER, H;WEILER, EW
通讯作者：
WEILER, EW