Intravenous administration of human adipose-derived stem cells ameliorates motor and cognitive function for intracerebral hemorrhage mouse model
Intravenous administration of human adipose-derived stem cells ameliorates motor and cognitive function for intracerebral hemorrhage mouse model
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DOI：
10.1016/j.brainres.2018.12.042
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发表时间：
2019-05-15
期刊：
影响因子：
2.9
通讯作者：
Yoshimura, Shinichi
中科院分区：
文献类型：
--
作者：
Kuramoto, Yoji;Takagi, Toshinori;Yoshimura, Shinichi
即使在今天，脑出血仍然是导致死亡和残疾的一个主要原因。康复治疗优先应用于功能恢复，但其效果有限。最近的研究表明，静脉注射间充质干细胞可以改善脑出血后的神经功能缺损。我们的实验室已经建立了人脂肪源性干细胞（hADSCs）。我们旨在通过临床相关的脑出血小鼠模型来评估hscs对脑出血后神经功能缺损的治疗效果。我们还评估了免疫反应，以阐明潜在的机制。hscs高水平表达MSC标记物。神经行为学实验表明，hADSCs可改善ich小鼠亚急性期的神经功能缺损。此外，hADSC使ICH病变中CD11（+）、CD45（+）细胞数量减少，CD86（+）、Ly6C（+）细胞比例增加。总之，急性期静脉给药hascs改善了亚急性期ich诱导的神经功能缺损，因为CD11(+)CD45（+）亚群介导的急性炎症受到抑制。我们的数据表明，hscs可以作为脑出血治疗的一种新策略。
Even today, intracerebral hemorrhage (ICH) is a major cause of death and disabilities. Rehabilitation is preferentially applied for functional recovery although its effect is limited. Recent studies have suggested that intravenous administration of mesenchymal stem cells would improve the post-ICH neurological deficits. Human adipose-derived stem cells (hADSCs) have been established in our laboratory. We aimed to evaluate the therapeutic efficacy of the hADSCs on the post-ICH neurological deficits using a clinical-relevant ICH mouse model. We also evaluated immune responses to clarify the underlying mechanisms. The hADSCs expressed MSC markers at high levels. The hADSCs administration into the ICH-bearing mice improved the neurological deficits during the subacute phases, which was shown by neurobehavioral experiments. Besides, the hADSC administration decreased the number of CD11(+)CD45(+) cells and increased the proportion of CD86(+) and Ly6C(+) cells in the ICH lesions. In summary, intravenous administration of hADSCs during the acute phase improved ICH-induced neurological deficits during the subacute phase because of the suppression of acute inflammation mediated by CD11(+)CD45(+) subpopulations. Our data suggest that hADSCs can be served as a novel strategy for ICH treatment.