Effect of an anti-lipoprotein lipase serum on plasma triglyceride removal.
Effect of an anti-lipoprotein lipase serum on plasma triglyceride removal.
复制标题
抗脂蛋白脂肪酶血清对血浆甘油三酯去除的影响。
DOI：
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发表时间：
1976
影响因子：
6.5
通讯作者：
M. W. Yang
中科院分区：
文献类型：
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作者：
I. Kompiang;A. Bensadoun;M. W. Yang
静脉注射抗脂蛋白脂酶血清定量阻断极低密度脂蛋白（VLDL）甘油三酯的催化作用。在用从鸡脂肪组织制备的高度纯化的脂蛋白脂肪酶（LPL，甘油酯水解酶，EC 3.1.1.3）免疫的兔中产生抗体。抗LPL血清注射后，血浆甘油三酯浓度呈线性增加。根据注射抗LPL血清的动物血浆中甘油三酯蓄积的速率或生物标记VLDL的消失曲线估计血浆中甘油三酯的进入速率。在相同动物中进行两次测量的情况下，两种程序之间非常接近一致。抗LPL血清对VLDL甘油三酯催化活性的抑制提供了一种表征新分泌的VLDL的方法，该VLDL表现出宽范围的颗粒大小，中值为625 A度。它们含有76.2 +/- 1.2%的甘油三酯，游离胆固醇与酯胆固醇的比率较高（2.46 +/- 0.45）。在对照VLDL样品中，甘油三酯为46.1%，游离胆固醇与酯胆固醇的比值为1.19。对脂肪组织LPL制备的抗血清对甘油三酯去除的完全抑制表明，在肝素-琼脂糖凝胶和伴刀豆球蛋白A-Sepharose柱上通过亲和色谱法制备的NaCl抑制的血清活化的脂肪酶是负责体内VLDL甘油三酯的catenation的酶。此外，血浆中甘油三酯蓄积的动力学提供了VLDL甘油三酯降解位点位于血浆隔室内的证据。
Anti-lipoprotein lipase sera injected intravenously in roosters blocked quantitatively the catabolism of very low density lipoprotein (VLDL) triglyceride. Antibodies were produced in rabbits immunized with highly purified lipoprotein lipase (LPL, glycerol ester hydrolase, E C 3.1.1.3) prepared from chicken adipose tissue. Following anti-LPL serum injection there was a linear increase in plasma triglyceride concentration. The rate of entry of triglyceride in plasma was estimated from the rate of triglyceride accumulation in the plasma of animals injected with anti-LPL serum, or from the disappearance curve of biologically labelled VLDL. In instances where both measurements were conducted in the same animals there was very close agreement between the two procedures. Inhibition of VLDL triglyceride catabolism of anti-LPL serum provided a way to characterize newly secreted VLDL that exhibited a broad spectrum of particle sizes with a median of 625 A degrees. They contained 76.2 +/- 1.2% triglyceride and had a high ratio of free to ester cholesterol (2.46 +/- 0.45). In control VLDL samples there was 46.1% triglyceride, and the ratio of free to ester cholesterol was 1.19. The complete inhibition of triglyceride removal by an antiserum prepared against adipose tissue LPL demonstrates that the NaCl-inhibited, serum-activated lipase prepared by affinity chromatography on heparin-Sepharose and concanavalin A-Sepharose columns is the enzyme responsible in vivo for the catabolism of VLDL triglyceride. Further, the kinetics of triglyceride accumulation in the plasma provide evidence that the site of degradation of VLDL triglyceride is within the plasma compartment.