Removal of PSA from NCAM affects the survival of magnocellular vasopressin‐ and oxytocin‐producing neurons in organotypic cultures of the paraventricular nucleus-猫眼课题宝

从 NCAM 中去除 PSA 会影响室旁核器官型培养物中产生大细胞加压素和催产素的神经元的存活

DOI：

发表时间：

2003

期刊：

European Journal of Neuroscience

影响因子：

3.4

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

L. Vutskits;E. Gascon;J. Kiss

下丘脑-神经垂体系统中聚唾液酸神经细胞粘附分子（PSA-NCAM）的表达与形态功能可塑性相关。在这项研究中，我们研究了 PSA-NCAM 在该系统中产生催产素 (OT) 和加压素 (VP) 的巨细胞存活中的作用。我们使用了最近开发的大鼠下丘脑室旁核（PVN）的器官切片培养模型，其中睫状神经营养因子（CNTF）和白血病抑制因子（LIF）是大细胞神经元的有效生存因子。我们通过共聚焦显微镜证明培养的大细胞 VP 和 OT 神经元对 PSA-NCAM 表达强烈的免疫反应性。在低浓度 CNTF 存在的情况下，Endo N 酶从 NCAM 中去除 PSA 会导致 VP 和 OT 神经元显着损失。在 LIF 存在的情况下，Endo N 处理不会改变细胞存活率。这些结果表明，除了在神经胶质可塑性中的作用外，PSA-NCAM 还可能影响下丘脑 VP 和 OT 神经分泌细胞的营养因子反应性。

The expression of the polysialic acid neural cell adhesion molecule (PSA‐NCAM) in the hypothalamo‐neurohypophyseal system has been correlated with morphofunctional plasticity. In this study, we investigated the role of PSA‐NCAM in the survival of oxytocin (OT)‐ and vasopressin (VP)‐producing magnocellular cells of this system. We used a recently developed organotypic slice culture model of the rat hypothalamic paraventricular nucleus (PVN) in which ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) are potent survival factors for magnocellular neurons. We demonstrate by means of confocal microscopy that cultured magnocellular VP and OT neurons express strong immunoreactivity for PSA‐NCAM. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons in the presence of low concentrations of CNTF. Endo N treatment did not change cell survival in the presence of LIF. These results suggest that, in addition to its role in neuro‐glial plasticity, PSA‐NCAM might also influence the trophic factor responsiveness of hypothalamic VP and OT neurosecretory cells.

Use of prokaryotic-derived probes to identify poly(sialic acid) in neonatal neuronal membranes.